51 
!No. 57. June 5. Infection experiment upon chinch-bugs ob¬ 
tained from Litchfield, iu Montgomery county, infected by^means 
of spores from an agar culture (No. 13) of Sporotrichum. Thirty 
bugs were treated and placed on growing corn under a bt -11 glass. 
June 7 two of the insects were dead and covered with Sporotri- 
chum, as well as one in the check; and two days later others had 
died in both lots. Ly the 11th, nine of the thirty had perished 
with the fungus in the experimental lot and six in the check. 
It is evident that these bugs were diseased when collected, or 
else that the check had become infected from the neighboring’ex- 
periments. ^ 
/ o N °/ 58 ' 7 JuT]e 6 ’ Infection experiment upon grain plant-lice 
(biphonophora aver ce) collected from wheat heads, and treated 
with Sporotrichum spores from agar culture No. 13. The spores 
were dusted upon the surface of rain water, and into this the 
wheat heads.were dipped, with the plant-lice on them. The wheat 
was then placed on damp earth in a glass cylinder breeding-cage, 
oix days later, the wheat was much shriveled and slightly covered 
with mould. A few dead aphids, but no traces of Sporotrichum 
seen. June 14, all the aphids dead, and the greater part of them 
covered with the white fungus. Specimens preserved. 
An equal number of grain plant lice were separated and kept 
under similar conditions as a check upon this experiment. These 
were all dead by June 14, but there were no traces of the white 
muscardme among them, and none developed later 
Experiments 62 and 63 are identical with 58; but had a less 
marked result, only a single plant louse being found dead with an 
appearance of fungus disease. 
No. 59. June 8 . Infection experiment upon young chinch.bugs 
collected at Garble, dusted with spores from agar culture No 13 
and placed on growing corn in breeding-cage. The insects re¬ 
mained in good condition until June 19, at which time one was 
dead and covered with the white fungus. June 20 , several other young 
bugs from the same source as the above were treated with spores 
or Sporotrichum from dead chinch-bugs imbedded in 1 his same 
ungus collected at Litchfield, June 2 , and placed in this cage. 
One young bug dead with this fungous*disease was removed June 
29; and one more was taken out July 3; and still others were dead 
July b, and covered with the fungous growth. 
The check, in the meantime, remained in good condition and 
without loss. 
^° s ’ Culture experiments upon coagulated 
material filtered from beef broth, and on the beef itself after the 
roth had been extracted. The media were placed in common 
saucers, dusted with spores from agar culture No. 13, and covered 
t g ass p ares. Both were carefully examined each day until 
Ju £ e 2 io Wltb ? u ^ aD y trace of the Sporotrichum. 
will OI xr mu'* Ju P e 16-18. Infection experiments identical 
with No. oo. I he grain lice (Siphonophora, avevce) treated in the 
same manner and from the same source of infection (No. 13). 
No results, except that one louse was dead with the fungus June 
