53 
rapidly from day to day, spreading over the surface of the medi¬ 
um, and even penetrating the cracks and crevices where the meal 
had shrunken from the tube. A granular appearance was quite 
marked on the 17th, and spores were fully formed on the 22d. 
The surface had the characteristic yellowish appearance of matu¬ 
rity by the 23d; and the spores were easily detached by shaking 
on the 24th and 25th. 
No. 69. July 8. Infection experiment with cabbage worms (Pieris 
rapee), precisely similar to No. 36. The infection, however, in this 
experiment was derived from the preceding corn-meal culture 
(No. 68), and from a bran culture (No. 77) recorded farther on. 
One hundred worms were dusted with spores and placed on cab¬ 
bage growing outdoors, which was then enclosed under a net- 
covered frame. The worms were somewhat restless the following 
day, and one pupated on the 10th. Several were sluggish, of a 
daik appearance, and not disposed to eat. Ants (Lasius) had at¬ 
tacked two larvae that had fallen to the ground. July 11, twenty- 
two larvae had pupated, and the others were all dead or dying. 
Ants still eating those on the earth. Put ae, larvae, and fragments 
placed on damp earth under a bell-glass. July 16, several butter¬ 
flies emerged, and a number of hymenopterous parasites were re¬ 
moved on the 21st. Phorid ae developed in dead pupae and were 
preserved. No traces of Sporotrichum seen at any time in the 
cage, or on the pupae and larvae removed and kept on damp 
earth. The check of tventy worms remained in fair condition. 
No. 70. July 13. Infection experiment with chinch-bugs from 
Blue Mound, Illinois, treated with spores from cultures 68 and 72. 
Several hundred chinch-bugs were thoroughly dusted with Sporo¬ 
trichum spores, and placed in a small wooden box, together with 
a supply of previously moistened green corn leaves for food. The 
box was securely covered, and fresh food introduced when needed. 
July 14, no Sporotrichum seen. July 20, a few bugs dead with 
visible fungus; and still others on the 22d. July 24, the number 
of bugs with Sporotrichum compared with the live insects in the 
box was very small. July 26, twenty-one bugs are imbedded 
in the white fungus; the a;tack does not seem to increase very 
rapidly. July 28, no more dead insects seem. The cage completely 
overhauled the 31st, and twenty-four fungus-covered bugs counted. 
A few dead without any external fungous growth. August 3, a few 
more dead with disease. Several adults pairing; a few eggs found 
on corn leaves; pupae not very abundant. August 7, no more fun¬ 
gus seen. August 10, a piece of middlings culture from No. 41, 
well covered with white lungus in a fruiting condition, was placed 
in the cage. Four days later a few bugs were dead with the dis¬ 
ease. On the 16th, about the same number of dead were seen, the 
fungus was more abundant on the 19th, and little or no change 
was noted the 22d. Cage overhauled the 25tb, and specimens dead 
with the white fungus picked out and preserved. Less than half 
the bugs dead at this time, and not all the dead showed an exter¬ 
nal fungus giowth. August 31, a few more dead, but not much 
fungus visible. September 6, Sporotrichum more abundant, and 
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