54 
dead bags more numerous. Experiment closed September 14, num¬ 
ber of fungus-covered bugs slightly increased. 
No. 71. Juue L2. Culture experiment on sawdust mixed with 
beef broth, and sown with spores from the Thaxter agar culture, 
No. 7. T he medium was prepared precisely as in experiments 39 
and 40. No growth on the 14th; first growth noticed on the after¬ 
noon of Juue 15th. The fungus spread slowly and had penetrated 
the air spaces in the medium by the 17th; was very slightly gran¬ 
ular by the 20th; and faintly yellowish by the 22d; Little or no 
change on the 25th. A slow start, sluggish growth, and scanty 
development of spores. 
No. 72. June 12. Culture experiment like the preceding (No. 
71), varying ouly in the nutrient material used, which was a bat¬ 
ter of middlings mixed with beef brolh, in this respect identical 
with No. 41. Fungus spores from the Tiiaxter culture (No. 7) were 
used to infect the medium. The fungous growth was visible the 
second day, anti by the 18th had spread freely over the bid face and 
was becoming granular and yellowish. The tube was opened on the 
19th and part of the fungus was removed and used for infecting No®. 
73 and 74. That remaining in the tube continued to grow and 
completely covered the surface, spores being easily detached on 
the 24th. 
No. 73. June 19. Infection experiment with Colorado potato 
beetles treated with spores from culture No. 72. The Sporotrichum 
of this c dture was in a fruiting condition, bnt the spores were not 
easily detached by vigorous jarring. The beetles were infected by 
dipping them in water upon the surface of which the spores had 
been thrown, and were then placed in a breeding-cage with grow¬ 
ing potato plant for food. A check lot was placed in a similar 
cage, and observed until July 6. Results purely negative, no Sporo¬ 
trichum appearing at any time in either cage. 
No. 74. June 19. Like No 73, except that crickets (Gryllus) 
were the subjects of the experiment aud that they were iufected 
by rolling them about iu spores from culture No. 72. Maintained 
until July 6, with negative result. No Sporotrichum in either ex¬ 
perimental lot or check. 
The failure of the infection in this and the preceding case was 
probably due to the immature condition of the spores used for 
infection. . 
Nos. 75 and 76. July 10. Culture experiments on corn meal mixed 
with white and yolk of au egg. The nutrient material was 
placed in tumblers, dusted with spores from culture No. 72, and 
covered with glass. No fungous growth appeared. Experiments 
discontinued July 15. 
For other 'experiments with white aud yolk of an egg used as 
tie nutrient material see Nos. 43 and 41. 
No. 77. June 12. Culture experiment on bran mixed with beef 
broth, prepared aud sterilized as in Nos. 53 and 54. Infected 
with Sporotrichum spores from No. 7. The mycelial threads ap¬ 
peared on the surface of the medium two days later, grew rapidly, 
and had begun to form spores by the 18th. A yellowish tinge, 
