55 
characteristic of ripeness, was barely apparent on the 19th; and 
spores were very easily detached by slight jarring on the 25th. 
No. 78. July 7. Culture on corn meal and beef broth, infected 
with spores from the preceding bran culture, No. 77. Beef broth 
made by boiling one pound of beef in one quart of soft water for 
three hours. The broth was then filtered, and neutralized with 
carbonate of soda. A thick corn meal batter, made by mixing 
the broth and meal, was placed in fruit-jars with altered caps 
(see PL V., Fig. 1 ), sterilized, and treated with Sporotrichum spores 
July 8. Growth had begun July 10, and the surface of the medium 
was thoroughly covered on July 11. By the 16th, the surface was 
all covered, and by the 20th it had taken on the yellowish tinge 
of ripeness. Spores were easily detached on the 22d. 
No 70. June 15. Culture experiment on corn meal saturated 
with beef broth without resort to sterilization, infected with Sporo¬ 
trichum spores from No. 7. Meal spread evenly over the bottom 
of a shallow pau and thoroughly saturated with beef broth, which 
had been standing uncovered for three days, and hence was full 
of bacteria. Spores from the Thaxter agar culture were dusted 
over the surface of the medium, which was then covered with 
glass. A slight trace of the white fungus appeared Juue 16, but 
soon disappeared, and no further development of it was seen. 
Discontinued June 22. 
Nos. 80 and 81. June 16. Culture experiments in the open air 
on a mixture of corn meal and beef broth, aud corn meal and 
soft water, without the usual process of sterilization. The media 
were placed in tin pans, infected with spores from the Thaxier 
agar culture, No. 7, and then covered with glass. No Sporotri¬ 
chum appeared at any time, and both cultures were discontinued 
June 22. 
Nos. 82 and 83. June 17. Two open air culture experiments on 
corn meal saturated with modified Cohn’s solution,* and dusted 
with fungus spores from No. 7. Experiment 82 was acidulated 
with one-twentieth of one per cent, and No. 83 with one-tenth of 
one per cent, of acetic acid. The nutrient material was placed in 
glass dishes, sown with spores of the white fungus, and then 
covered with a glass plate. Both masses were covered with molds 
on the 19th, and the experiments were discontinued on the 20th; 
no Sporotrichum having been seen. 
No. 81. December 21. A test-tube culture experiment on corn- 
meal saturated with beef broth prepared as in No. 78, sterilized, 
and infected with spores of Sporotrichum from the Thaxter agar 
culture, No. 7. A slight mycelial growth appeared the 22d, but 
was not Sporotrichum. Several other fungi appeared in this tube, 
but no white muscardine up to January 15, 1892. The culture 
was again examined July 7, with the same result. 
No. 85. June 1. A contagion experiment with live chinch-bugs 
from Litchfield, Illinois, confined with chinch-bugs dead with 
•Distilled water 42.315 gramme*, caae sugar 7.000 gramme*, ammonium tartrate .250 
grammes, potassium phosphate ,125 gramme*, magnesium sulphate .125 grammes, cale.um phos¬ 
phate 125 grammes. The purpose of this solution is to afford a suitable nitrogenous lood for 
the growth of the tungus aud at the same time to check bacterial development and the conoeuuent 
appropriation of the food prepared for the fungus. 
