56 
Sporotrichum disease, from experiment No. 2. Thirty chinch-bugs 
were placed in a breeding-cage, and exposed to infection forty- 
eight hours, from four fungus-covered bugs. June 10, three dead 
and covered with the fungus. All the whitened bodies removed 
and placed in a bottle. The live bugs remaining in the cage were 
now used for other experimental purposes. 
In check lot, established the same time, no deaths. 
No. NO June 8. An infection experiment with young chinch- 
bugs, just past the second molt, dusted with spores of Spoiotri- 
ebum, and then enclosed in a breeding-cage. The young bugs 
were treated with spores by being well shaken in a bottle, into- 
which several bugs dead with the fungos of the white muscardine 
had been previously placed. Second infection, from other dead 
chinch-bugs, June 20. The experiment was a failure, as none of 
the young bugs were attacked by the fungus by June 10, and the 
cli eck lot. alho remained without loss. 
The diseased bugs used iu this operation came from a stock 
cagn kept in the in sectary, supplied with chinch-bugs collected 
from wheat fields at Litchtield, June 2. June 5, several bugs in 
this cage were dead with white lungus, and the fungus attack in¬ 
creased in intensity up to, and including June 29. In the mean¬ 
time, the fungus-covered bugs were removed and used for experi¬ 
mental purposes. The appearance of Sporotrichum in this cage 
was due to the presence of the spores when the insects were col¬ 
lected, or else they became infected from neighboring experimental 
lots. The former, however, seems quite probable, as dead chinch- 
bugs imbedded in this fungus were taken from the wheat at the 
time the collection was made. 
N >. h7. Jwnti 1'. A test-tube culture experiment on corn meal 
and b^et brodi, prepared like No. 78, and at the same time. The 
medium was infected with spores from a chinch-bug dead with 
white muscardine, originally from the same source as those used in 
80. The fungus-covered bug was dropped into a tube, where it 
remained upon the surface of the culture for a short time, and 
was then removed. Two days later a slight mycelial growth of 
the white muscardine appeared, spread rapidly, and^almost com¬ 
pletely covered the surface of the medium by the 17th. A blue 
mold appeared iu the bottom of the tube at this time. June l8 r 
Spoiotrichum same as yesterday; blue mold spreading. June 
20, Sporotrichum spore-clusters beginning to form. June 22, the 
fungus iiad the characteristic yellowish appearance of maturity. 
June 24, blue mold appeared in the upper part of the tube. 
Sporotrichum thoroughly ripe and in good condition June 27. 
Culture affected bv bacteria, producing a reddish discoloration 
about and beneath the moldy spots. 
Nos. 88, 89, 90, aud 9L June 12, and 30. These are successive 
test-tube culture experiments on bran, middlings, saw-dust, and 
corn meal mixed with beef broth, prepared aud sterilized like No. 
68. The fungus covered bugs used to infect these tubes came 
from the same source as those used in 86 and 87. The bugs were 
placed in the tubes and rolled over the surface of the media for 
