57 
a few minutes and then removed. Sporotrichum failed to germ¬ 
inate, and the tubes were filled with a profuse growth of mold. 
No. 92. June 30. A tnst-tube culture on corn meal mixed 
with beef broth as in No. 68. A chinch-bug dead with Sporotri¬ 
chum, originally from Blue Mound, Illinois, was used to infect 
this tube. The fungus, however, was in a fruiting condition, but 
the spores were not thoroughly ripe. A feeble growth of Sporo¬ 
trichum appeared July 3, but was soon crowded out by a profuse 
growth of mold which took possession of the medium. 
No. 93. July 3. Test-tube culture experiment on corn meal mixed 
with beef broth, sterilized at a temperature varying from 100 to 
107° Cent, for thirty-five minutes. Two tubes weie prepared and 
treated with fungus spores from beetles (Lachnosterna and Dia- 
brotica) and a myriapod found in earth near Urbana, Illinois, June 
2, 1891. The spores were introduced into the tube by means of 
a sterilized platinum wire. A slight mycelial growth of the white 
muscardine appeared on the surface of each culture July 5. 
Sporotrichum was spreading over the medium on the 8th, but other 
fungi had appeared and were also spreading. Sporotrichum was 
mote plentiful the 10th, and other fungi growing about as rapidly. 
By the 18th, the intruding fungi had crowded out the white fun¬ 
gus in one tube and by the 2ith had completely choked and 
hidden it in the other. 
No. 91. July 16. A test-tube culture on a mixture of corn 
meal and beef broth, prepared and sterilized as for No. 93. The 
fungus spores used in this experiment came from thistle cater¬ 
pillars (Pyraweis carrhii), collected from thistle (Cirsinm lanceo- 
lalum) near Urbana, June 30, and placed in a breeding-cage in 
the insectary. The fungus of white muscardine appeared spon¬ 
taneously ou both larvae and puj se of this lot about July 8. 
Sporotrichum had begun to grow ou the culture medium July 18, 
and increased rapidly, having spread over the greater part of the 
culture surface two days later, The growth was quite granular, 
and of a yellowish tiDge (indicating ripeness) by the 24th. Spores 
from this tube were used in the successful culture immediately 
following (No. 95), and in subsequent culture experiments made 
in 1892 (Nos. 2, 3 and 26; Jin. 5, March 12, 22). 
No. 95. December 21. A t^st-tube culture from No. 94 on a 
batter of corn meal and beef broth prepared like No. 94, except 
that the tube was sterilized for three-quarters of an hour at a 
temperature of 1U0 Cent. A profuse growth of Sporotrichum ap¬ 
peared two days later, and by the 28th had covered the greater 
part of the nutrient material. A few spores were beginning to 
form on the 30th, and by January 4 (1892), the characteristic yel¬ 
lowish tinge, indicating ripeness, appeared. A few spores were 
easily detached by shaking. By the 11th, a fruiting growth of 
Sporotrichum had completely covertd the entire surface of the 
medium. 
No. 96. July 21. A contagiou experiment with chinch-bugs 
exposed to infection by means of fungus-covered chinch-bugs re¬ 
ceived from Dr. Snow July 18.* Several hundreds of bugs col- 
