58 
lected at Litchfield July 18 were placed in a tight wooden box 
with the dead chinch-bugs and supplied with fresh food from time 
to time. The first dead bug in this lot was seen August 3, and 
gave no external evidence of infection. August 16 two were seen 
in this cage covered with Sporotrichum. A few other whitened 
bodies were found on the 19th, 22d, and 25th. Between the latter 
date and the 31st little or no change took place. A slight ap¬ 
parent increase of the infection was noted September 6, but there 
was no further progress of the disease appaient by the 30th of 
that month. The box was then restocked with about two hun¬ 
dred and fifty live chinch bugs originally obtained at Litchfield 
September 23. Nothing further was noted in this box to indicate 
a progress of disease until December 8, when a few bugs were 
seen with a fresh growth of Sporotrichum on their bodies. 
No. 97. July 21. A con agion experiment arranged precisely 
as the above (No. 96) except that the bugs used for infection 
(received also from Dr. Snow) had died as a result of parasitism 
by Empusa aphidis. In this box also no dead bugs were seen 
until August 3, and then only a few, without trace of any exter¬ 
nal fungus growth. On the 14th of August, a small number were 
seen with a fresh growth of Sporotricnum on their bodies, but 
none with Empusa. Several others dead at this time bore no exter¬ 
nal fungous growth. August 22 a considerable number of eggs were 
noticed in the cage, and more than half the bugs were dead, very 
few or them, however, with any external fuugous growth. Similar 
notes were made on the 31st of August and on the 6th and 14th 
of September. On the 29ih, only eighteen live bugs were found 
in the box, and on those dead there was no external growth of 
Empusa, a small number showing Sporotrichum only. 
A check on the two preceding experiments (96, 97) was separ¬ 
ated the same day, July 21, and kept under precisely similar con¬ 
ditions. One bug dead with Sporotrichum was removed August 3. 
A few adults were seen pairing, and several eggs were found on 
leaves, some of them shriveled and dried up. No fungus on any 
of the enclosed bugs at any later date. The spontaneous appear¬ 
ance of Sporotrichum in this box shows that it was brought with 
them from the field, or that they became infected from other ex¬ 
periments in the same building. 
No. 98. August 7. A chiuch-bug contagion experiment with 
live chinch-bugs received from Dr. Snow on this date. About 
seventy-five live bugs were placed in a small sack of Swiss muslin 
and supplied with green corn leaves for food. The bugs dead 
with Empusa, used in the preceding experiment (No. 97), were 
removed from that cage and placed in the sack just mentioned, 
which was then enclosed in contagion box No. 97, Fresh food 
was introduced into the sack when needed. One dead bug, show¬ 
ing an external growth of Sporotrichum, was seen August 15, 
several others had died and were covered with the same fungus 
the 17th, and still others were seen the 22d and 31st. Only 
fifteen live bugs remained in the cage September 14, and these 
were all dead, except one, on the *28th. The fungus-covered bugs 
