59 
were removed August 22 and September 14. Those taken out on 
the former date contained an unmistakable growth of Sporotrichum, 
while those removed the latter date were imbedded in a mixed 
growth which was not definitely determined. 
Unfortunately, however, for the significance of these results, 
about half the specimens from the check originally separated, had 
perished with the Sporotrichum disease, thus giving evidence 
either that the original lot from Dr. Snow had been diseased 
when taken, or else that the check had become infected from the 
neighboring experimental lots. 
No. 99. July 21. A contagion experiment precisely similar to 
No. 98, except that the infection used consisted of bugs said to 
have died from a “mixed disease” (Sporotrichum and Empusa), 
-which were received from Dr. Snow on the same day as those 
used in the tvo experiments immediately preceding. Fresh food 
was introduced into the cage when needed. A few c^ead bugs 
were seen July 28, but none with fungus. August 3, cage over¬ 
hauled. A few bugs seen pairing and several eggs found on 
leaves. A f«w dead with fungus, probably Sporotrichum. Two 
more bugs dead with the fungus were removed on the 7th. Sev¬ 
eral with a fresh fungous growth on their bodies were noted on 
the 14th, and still others on the 16th, 19th, and 22d. By the 25th, 
nearly three fourths of the bugs were dead, and the great majority 
■of them were covered with the white fungus. More bugs dead 
with fungus were seen September 6, and others on the 14th. 
Only four live bugs remained the 25th. A careful examination of 
the fungus-covered bugs removed showed that Sporotrichum was 
the only fungus present. The check upon this experiment was 
the same as that for Nos. 96 and 97. 
No. 100. August 7. A contagion experiment precisely similar 
to No. 98, except that the diseased bugs used came from experi¬ 
ment No. 99. Fresh food was introduced into the sack when 
needed. A few bugs dead with Sporotrichum were seen on the 
17th, and about two-thirds of the entire lot were dead by the 22d, 
one-half of them being covered with fungus. Eggs were seen in 
small numbers, and two iinagos in coitu were found dead with the 
white fungus. Still others were dead the 25th, and all had died 
by September 9, most of them imbedded in Sporotrichum. 
The check upon this experiment was the same as that for No. 
98, and the results obtained are therefore not very significant, as 
it is quite likely that the live bugs used were diseased when 
received. 
No. 101. December 22. A test-tube culture on corn meal and 
beef broth mixed to a thick batter, sterilized for three quarters 
of an hour at a temperature of 100 Cent., and then treated with 
Sporotrichum spores from the femur of Pezotettix differentialis, 
originally found at Urbana, Illinois, September 16. There was a 
visible Sporotrichum growth in the tube December 24, and the 
surface of the medium was completely covered four days later, at 
which time other fungi made their appearance in the tube. These 
finally took possession of the medium, and by January 14, 1892, 
had suppressed the Sporotrichum and filled the tube. 
