61 
cultures 52 and 54, which gave again the B >trytis form usual in 
artificial cultures. This fact was further verified by repeated cub 
tures from these tubes, namely, 53, 55, 5J, and 57, all of which 
consisted wholly of unmistakable Sporotrichum globuliferum. 
As a result of the laboritory infection experiments with Sporo- 
trichum on the chinch-bug. it appeared that while most of these 
were successful, several of them were only doubtfully so, the in¬ 
sects treated dying in coufiuement but without any subsequent 
external fungous growth * The method used was imperfect, how¬ 
ever, as these dead specimens should have been kept for four or 
five da\s on damp sand in a damp atmosphere and at a suit¬ 
able tempt rature. 
THE FIELD INFECTION EXPERIMENTS. 
Our experiment with Boivyhs ienella all depend upon three 
tubes of that fungus received from MM. Fribourg & Hesse, of 
France, a firm of chemists who advertised this fungus for sale'as 
an insecticide especially recommended for the destruction of the 
ver blanc , representative, in the agriculture of Europe, of the 
white grub of America. The spores from which their cultures 
were made were understo >d co have been obtained from Prof. A. 
Giard, of Paris. The material came to us in glass tubes stopped 
with cotton at one end, the first of them January 5 and the two 
others February 19. In addition to the fungus spores, these tubes 
evidently contained a good deal of foreign matter resembling meal, 
which however we did not attempt to determine. The mass was 
also impure from a botanical standpoint in all the tubes, contain¬ 
ing a considerable quantiiy of blue mold, and presenting reddish 
discolorations in spots, as if by a bacterial infection. The results 
of our expenments show, however, that the tubes also contained 
living spores of Bolrytis ienella. The tubes received in February 
were reported by the senders to have come from cultures freshly 
made that year. 
A tube received May 25 from Prof. Thaxter—itself a culture 
from material obtained from Prof. Giard, of Paris—was used by 
us only as a starting point for cultures 7b and 79 of the following 
list. 
All our cultures from the first tube received, that of January 5, 
were failures, owing to the extreme impurity of the growths. In 
some cases a mycelium grew which presented the general ap¬ 
pearance of Boirylis ienella, but it was speedily overcome by more 
vigorous fungi, and was suppressed without fruiting. There can 
be no question, however, that the Boirytis spores were present 
even in this first sending, since infection experiments, although not 
entirely satisfactory, resulted in a few cases in the killing of larvae 
with the subsequent reddish discoloration characteristic of the action 
of this fungus. The second sending was more satisfactory, although 
still impure. Growths of Botrytis appeared in some of the tubes 
in sufficient abundance to hold their own, and by transfer of these 
* For further light on the cause of such losses of huge not visibly infected, see experiment 72, 
series of 1894. 
