62 
to other tubes fairly clean cultures were obtained. Infection ex¬ 
periments with this better material were successful on white grubs 
and Bibio larvae, and possibly upon chinch-bugs also. In general, 
however, this Botrytis compared unfavorably with Spo rot rich um 
so far as our experiments extended 
March 21, a tube of lsaria leprosci was received from Prof. 
Thaxter, with the suggestion that it be tested as an insecticide. 
This tube —whose previous history is unknown to me—was used 
only as a source of infection for several cultures made in the 
bacteriological laboratory of the University, and from these our 
experimental material was wholly drawn. Infection experiments 
with these cultures promise better results for this fungus than for 
Botrytis tenellci. Two such experiments on white grubs and one 
on caterpillars of Lepidoptera had a successful issue, while an in¬ 
fection of chinch-bugs was apparently without result. 
CLASSIFIED LIST of experiments— 1892. nos. 1—86. Experiments 
with Sporotrichnm Glohuliferum. 
Cultures on agar-agar: 
No. 3. March 22. From corn-meal culture of 1891 (No. 94). 
No. 
No. 
No. 
No. 
No. 
No. 
No. 
No. 
No. 
No. 
No. 
No. 
No. 
No. 
69. March 22. From original Thaxter culture (No. 7, 1841). 
70. March 24. From original Thaxter culture (No. 7, 1891). 
71. May 5. From dead chinch-bugs. 
72. May 11. From dead chinch-bugs. 
76. . May 17. From Euschistus dead in field. 
77. November 17. Agar culture from No. 76. 
52. July 8. From white grub experiment, No. 51. 
5fc. Julv 15. From white-grub experiment, No. 51. 
From No. 54, second remove from grub. 
From dead chinch-bugs; northern Illinois. 
November 15. Spores from dead beetles found in the 
field November 8. 
56. October 31. From No. 55, third remove from grub. 
53. November 17. From No. 52, second remove from grub. 
57. November 17. From No. 56, fourth remove from grub. 
55. 
82. 
8 ±. 
July 
July 
July 
15. 
15. 
20 . 
Cultures on corn-meal mixtures: 
No. 26. March 12. Fruit-jar culture. Spores from 1891 series 
(14, 31, and 94). Second remove from insect. 
No. 27. March 14. Fruit-jar culture. Spores from 1891 series 
(14, 32, 47, 59, and 94). Second remove from insect. 
No. 49. March 14. Test-tube cultures from 1891 series (7 
and 14). 
No. 50. April 11. Fruit-jar culture. Spores from No. 49. 
No. 73. July 7. Fruit-jar culture from agar culture 72, second 
remove from chinch-bug. 
Cultures on flour paste: 
No. 1. January 5. From series of 1891 (Nos. 31, 47, and 50)^ 
No. 2. January 5. From series of 1891 (No. 94). 
