68 
DETAILED DESCRIPTION OF EXPERIMENTS, 1892. 
No. 1. January 5. A fruit-jar culture on wheat flour and beef 
broth, and corn meal and beef broth, so managed as to afford a 
large surface area in a small space. A half-gallon jar was first 
•coated inside with a thin paste of flour and water, which was 
.allowed to dry, and was then given a second coat of flour paste 
to which a little beef broth had been added. Several tin-box lids 
strung on a wire, filled with a batter of corn meal and beef broth, 
were then suspended from the cover, after which the jar was 
closed and sterilized for fifty minutes in boiling water, and infected, 
through the tube, with spores from cultures 31, 47, and 50 of the 
series for 1891. 
January 11. A good growth of Sporotrichum covered the corn 
meal, but was less vigorous on the wheat flour on the sides of 
the jar. Two days later the fungus in the cup* was in fruit, but 
the precise condition of the growth on the side of the jar could 
not be ascertained, owing to the opacity caused by the paste. 
Bacteria appeared in the jar January 16, and four days later had 
spoiled the experiment. 
No. 2. January 5. A test-tube culture on a paste of wheat 
flour and beef broth sterilized at a temperature of 100 Cent, 
and treated with fungus spores from corn-meal culture No. 94, 
started July 16, 1891. By January 11 Sporotrichum was quite 
abundant on the thicker parts of the medium; but the growth 
had become impure by the 16th. It was not at any time so pro¬ 
fuse as that od corn meal and beef broth. Wheat flour was not 
regarded as a good material for these cultures. It will be noticed 
that the spores for this infection were more than six months old. 
No. 3. March 22. A test-tube culture on agar prepared accord¬ 
ing to the usual formula, and treated with spores from corn-meal 
culture No. 94, series 1891, now almost eight months old. This 
old culture mass had become dry and hard, and rattled against 
the sides of the tube like a piece of bone. Its spores were, how¬ 
ever, in good condition, and an abundant mycelial growth was 
plainly visible in the new tube March 25, three days from the 
time of sowing. This was still increasing on the 28th, and spores 
were beginning to form on the 29th. The Sporotrichum continued 
to grow, and the spores had begun to ripen April 5. Many were 
easily detached by shaking April 8, and all seemed ripe by the 
15th. 
No. 4. January 11. A test-tube culture on corn meal and beef 
broth sterilized for forty minutes at a temperature of 100 Cent., 
and infected with spores of Botrytis ienella from the Fribourg <fc 
Hesse culture previously described (see p. 61). The infection was 
made by quickly removing the cotton plug of the tube and trans¬ 
ferring the spores on the end of a platinum wire which had been 
recently heated to redness. A white mycelium appeared promptly, 
and was already quite abundant by January 13, but greenish and 
pinkish tinges appeared in it the following day, indicating an 
impure culture. A slimy mold also sprang up, which by the 20th 
