69 
had practically suppressed everything else in the tube. When the' 
culture was thrown out, January 25, no trace of Botrytis was to- 
be detected in it. 
No. 5. January 11. Two test-tube culture experiments on agar, 
prepared exactly alike and at the same time, sterilized and treated 
with spores of Botrytis from the Fribourg & Hesse culture. A 
white mycelium was visible the following day, which had begun 
to fruit by January 13, presenting the general naked-eye charac¬ 
ters of Botrytis. It was evident, however, the next day that the 
culture was impure, a greenish fungus and a reddish bacterial 
contamination appearing. The culture was kept until the 30th 
and then rejected as without value. 
No. 6. March 19. An agar tuba infected with spores from No. 
5, from a portion of tha growth taken at the tima to ba Botrytis 
tenellci. The culture resulting proved, however, to ba a green 
mold, which was followed later by another foreign growth. 
Examination on the 30th showed no Botrytis tenella in this tube. 
No. 7. April 2L An agar culture with spores from the cul¬ 
ture immediately preceding. Five days later a greenish fungous 
growth had fruited, but no Botrytis was seen. Tube emptied on 
the 30th; still no Botrytis. 
No. 8. February 22. A test-tube culture on corn meal and 
beef broth sterilized for half an hour at 100 Cent., and treated 
with spores of Botrytis tenella from the culture received from 
Fribourg & Hesse, February 19. A slight mycelial growth 
was seen February 24, which had increased considerably by 
the 26th, and formed white tufts over the surface of the medi¬ 
um. This developed later into a blue mold, and further evi¬ 
dence of a mixed contamination presently appeared. The culture 
was, however, allowed to continue until the 20th of March, at 
which time its various fungous growths were microscopically 
examined. There was no Botrytis tenella in this tube. 
No. 9. February 22. A test-tube culture on agar sterilized 
and treated with spores from the same source as those used in 
experiment 8. Like the preceding, a mixed growth of various 
fungi, among which no Botrytis tenella matured. 
No. 10. February 28. Culture experiments on raw potatoes 
treated with spores of Botrytis tenella from the same source as 
those used in the two preceding experiments. The spores were 
dusted over the cut surface of raw potatoes, and kept under bell- 
glasses placed over dishes containing water. Results purely nega¬ 
tive. 
No. 11. March 11. An infection experiment upon Bibio larvae 
dusted with spores of Botrytis tenella. A large number of larvae 
were placed in a shallow tin pan and dusted with the contents of 
one of the tubes received from Fribourg & Hesse. The pan was 
then covered with a moist paper and another pan. Nothing of 
importance was noted until the 20bh, at which time the larvae were- 
beginning to pupate. On the 30th one larva showing a white 
mycelial growth and a pupa of a pinkish tint were dead. There- 
were several dead larvae in the cage at this time, but no others 
