71 
flame oE an alcohol lamp. The Botrytis, which had begun to grow 
May 9, developed freely, and May 11 was coloring the agar red. 
It was in a fruiting condition May 21, and bore an abundance of 
thoroughly ripened spores by the 25th, some of which were used 
in experiments 14 and 16. 
No. 14. June 6. Test-tube cultures on agar treated with spores 
from agar culture No. 13. Mycelial growth showed plainly two 
days later, and the agar already had. a reddish tint. The fungus 
spread rapidly and had begun to fruit by the 14th, at which time 
the greater part of the agar had become dark red. Spores ripe on 
the 18th and the agar losing its reddish color. 
No. 15. June 29. An agar culture infection from No. 14. Vis¬ 
ible growth July 2, small red spots in agar around the tufts of 
mycelium. July 7, agar nearly all red, with dark brownish spots 
where the mycelium is attached. July 9, red color of the agar 
had begun to fade, and by the 11th it had all disappeared. The 
fungous growth in the meantime had assumed a peculiar grayish 
tint, indicative of ripeness of the spores. 
No. 16. July 6. Agar cultures in six tubes infected from JN o. 
13. July 7, no visible growth. July 9, a white mycelium 
has started. July 11, mycelium spreading rapidly; agar entirely 
red. July 14, color unchanged; mycelium pure white and dense. 
July 15, spore heads visible. July 18, color of agar beginning to 
fade. On the 23d, spores ripe; red color of agar practically all 
gone. . , , 
No. 17. August 8. An infection experiment upon tomato 
worms (Protoparce) treated with spores of Botrytis from agar 
culture 16. Eight larvae were treated by rubbing the spores 
into the spiracles with a small brush, and placed in a breeding- 
cage. They all entered the ground on the 15th to pupate, and the 
moths emerged a little later. No fungus appeared. 
No. 18. August 15. An infection experiment with Botrytis 
tenella on chinch-bugs, the latter obtained from Forreston, Illi¬ 
nois, August 13. Several hundred live bugs were shaken up in a 
test-tube agar culture (No. 16) until their bodies were covered 
with spores. They were then put into a wooden box, supplied 
with food, and set on damp sand in a shady place in the insectary. 
None dead on the 18th, but several pupae had transformed to the 
imago. By the 21st several bugs had died, and a few showed a 
mycelial growth upon the surface, which proved, however, on 
microscopic examination to be a common mold bearing no re¬ 
semblance to Botrytis. More bugs died on the 29th, but no 
Botrytis tenella in the cage. Experiment abandoned. . 
No. 19. August 26. An infection experiment with white grubs 
dusted with spores of Botrytis from culture No. 16. Nineteen grubs 
were well dusted with spores and placed in a breeding-cage with 
grass roots for food. No fungus had appeared by September 4, 
and the grubs were in good condition. One was dead the loth, 
but contained no fungous growth. Ten w r ere dead the 24th, and 
four had Botrytis on their bodies in a fruiting condition. The 
others showed a slight mycelial growth, but no spores. The grubs 
