72 
were all dead by the 28th, bat only one exhibited the fungus in 
fruit. Six had no fungus on their bodies, and had probably died 
from some other cause. Several grubs kept in a stock cage near 
by died apparently in the same way as those last mentioned, ani 
no fungus appeared on their bodies. 
No. 20. October 31. A test-tube culture on agar infected from 
No. 16. Faint growth in a few spots November 7. Growth slow, 
barely showing white here and there November 12. November 
14, agar now colored red over about two thirds of the surface. 
No. 15, agar wholly red. November 19, mycelium spreading, but 
no other change. November 25, red of agar nearly all gone, a 
portion of the mycelial mass of a creamy yellowish color, and 
bearing spores. 
No. 21. May 25. An infection experiment with Cyclocephala 
and Lachnosterna larvae dusted with spores from one of the 
Fribourg & Hesse cultures received February 19. Fifty larvae 
were dusted with Botrytis spores, placed in a breeding-cage on 
damp sand aud covered with blue grass sod, where they were left 
without further inspection uatil June 29. Oa this date three 
grubs, two pupae, and eleven imagos were found dead. One dead 
larva had a slight mycelial growth on it. Dead larvae removed 
and placed on damp sand. July 8, one larva had developed on 
its body a considerable fungous growth which was in a fruiting 
condition. The experiment immediately following was begun with 
spores from this grub. 
A lot of fifty grubs was kept under similar conditions as a 
check. Several dead larvae, pupae, and imagos were found among 
them June 29, and were placed on damp sand. No fungus ap¬ 
peared. 
No. 22. July 8. A test-tube culture on agar infected with 
spores of the Botrytis fungus derived from a grub taken from ex¬ 
periment 21. The fungus started promptly, and was visible on the 
surface of the medium on the 10th, but bacteria appeared in the 
tubes and the growth became generally impure. The appearance 
of the characteristic reddish discoloration of the agar July 13, was 
finally the only evidence of the growth of the Botrytis tenella in 
these tubes. 
No. 23. July 13. An infection experiment with lepidopterous 
larvae and plant lice (collected by sweeping clover) dusted with 
Botrytis spores from one of the imported cultures received Feb¬ 
ruary 19. The insects were placet in a jelly-glass with clover 
leaves for food. Some of the plant-lice and two of the cater¬ 
pillars had died by the 25th; but no fungus appeared at aDy time 
in this cage. 
No. 24. October 31. An infection experiment with Anasa 
tristis and Euschistus, dusted with Botrytis spores from the 
Fribourg & Hesse culture. The spores were rubbed over the 
spiracles of some and dusted on others. The bugs had begun to 
die November 12; but no Botrytis appeared at this time or later, 
although the dampness was sufficient to permit the growth of 
common blue molds on their bodies. 
