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i 
No. 25. November 17. Test-tube culture on agar infected with 
spores from the same source as those used in No 24. Experiment 
continued until November 30, but only a green mold appeared 
in the tube. 
No. 26. March 12. Fruit jar cultures on corn meal and beef 
broth infected with spores of Sporotrichum from corn-meal 
cultures 14, 31, and 94 of 1891 series. Eight jars were filled and 
sterilized as usual, and treated with spores from the above sources. 
The fungus of the white muscardine started promptly; was in a 
fruiting condition thirteen days later; and had ripened its spores 
by April 3. As some of the jars were quite moist inside at this 
time, the caps were removed and cotton substituted. April 26 the 
spores were brushed from the surface of the nutrient material in 
five jars, and placed in a dry jar for future use. 
No. 27. March 14. Fruit-jar cultures of Sporotrichum on corn 
meal and beef broth similar to No. 26, except that the spores 
came from cultures 14, 32, 47, 50, and 94 of series 1891. Fourteen 
fruit-jars were prepared and infected. Seven days later, the jars 
infected with spores from culture No. 14 (June 4, 1891), which had 
been made more than nine months before, contained a profuse mycelial 
growth of the white fungus. The fungus failed to develop in five 
other jars, which were sterilized a second time and again infected 
with spores from cultures 31 aud 94 (1891 series) on the 17th. 
Six days later there was a fair growth of Sporotrichum on the 
meal. March 30 a greenish growth appeared m a few jars and 
somewhat retarded the development of the white fungus. This 
greenish fungus did not spread, however, after April 6. Sporotri¬ 
chum spores were very abundant April 4, and were fully developed 
in some jars two days later. Spores removed from four jars on 
the 26th, and placed in a dry jar for future experimental pur¬ 
poses. 
No. 28. March 30. An experiment to test the possibility of so 
thoroughly wetting the spores of Sporotrichum glohuliferum that 
they might be distributed in a spray. It became also incidentally 
an experiment on the beginnings of germination of the wetted 
spores. The fungus spores were taken from corn-meal cultures 
26 and 27 and dusted upon the surface of water in a large open- 
mouthed bottle. The water was then violently forced in and out by 
means of a syringe. This agitation was twice repeated, and half 
an hour later the spores were floating on the surface of the water, but 
so thoroughly wet that the least disturbance caused them to sink. 
An examination of the spores under a microscope a few hours later 
showed that they had already begun to grow, many of them having 
sent forth mycelial sprouts whose length was two or three 
times the diameter of the spore. On the 31st, growth was much 
more general and advanced, large numbers of the isolated spores 
having sent forth threads in two directions, and little clusters of 
spores bristling with growing hyphae Four days afterwards, little 
advance had been made, probably because the nutriment in the 
spores had been exhausted, while the fluid containing them 
yielded no nutriment. 
