74 
No. 29. April 7. An infection experiment with chinch-bugs 
treated with Sporotrichum spores from corn-meal cultures 2G and 
27. Fifty chinch-bugs were placed in water, the surface of which 
had been previously dusted with fungus spores. The insects were 
so thorough coated with spores that they had a whitish appear¬ 
ance when removed. They were then put into a pint fruit-jar and 
supplied with green wheat for food. No conspicuous fungus in¬ 
fection in this case during the entire month ot April. On May 2, 
eight dead bugs covered with Sporotrichum were removed, and 
several others imbedded in the same fungus were seen the follow¬ 
ing day. No trace of the disease was seen in the check at any 
time. 
No. 30. April 20. An infection experiment with chinch-bugs 
treated with Sporotrichum spores from cultures 26 and 27. Fifty 
chinch-bugs were well dusted with spores and put into a small 
wooden box, the inside of which had been previously treated with 
spores, with a supply of food. The box was then placed on 
damp sand and covered with a box of larger size. A few dead 
bugs were seen May 1, but no fungus was visible. Twelve days 
later two bugs covered with mycelium were seen, on which spores 
had begun to form by the 14th. Other bugs were dead and 
covered with the fungus on the 20th. All were dead by June 5, 
but less than two thirds showed any trace of Sporotrichum on 
their bodies. 
No. 31. May 4. An infection experiment with a miscellaneous 
lot of insects dusted with spores from cultures 26 and 27. . The 
insects were treated with spores, put into a half-gallon fruit-jar, 
and supplied with food. On May 8 they were transferred to a 
wooden breeding-cage. All had died by May 12, but no fungus 
had appeared. May 16 a bumble-bee showed an external growth 
of Sporotrichum about the head and thorax. 
No. 32. May 4. An experiment with larvae anl adult insects 
(Silphidm and Dermestidse) treated with spores from cultures 26 
and 27, and placed in a breeding-cage. Eight days later one 
silphid larva had died and one was covered with Sporotrichum. 
A few beetles were dead with the same fungus on the 16th. 
Nearly all were dead on the 28th, and most of them were covered 
with the fungus ot the white muscardine. Fungus spores from 
some of these beetles were used in the experiment next succeed- 
No. 33. June 20. A contagion experiment with Ischnodemus 
fattens (a bug closely related to the chinch-bug) treated with spores 
from a dozen beetles taken from the experiment next preceding, and 
then placed on damp sand under a bell-glass. Half a dozen bugs 
dead with this fungus were removed June 30, and all were dead by 
July 8, about one twentieth of them being covered with the 
fungus. A slender brown spider and a lampyrid beetle accidentally 
enclosed in the cage were found dead with the same fungus. _ 
A check on this experiment was established at the same time 
with insects ot the same species kept under similar conditions. 
