76 
The insects were treated with Sporotrichum spores and placed in 
a dry frnit-jar, which was then covered with gauze. The insects 
had all died by June 30 but no fungus appeared. 
No. 40. June 21. An infection experiment similar to No. 37. 
Bugs placed with grass in breeding-cage, and infected by dusting 
spores on earth and grass. The first fungus-covered specimen 
was seen July 1, and by July 8 all were dead. 
Check the same as that for No. 33. 
No. 41. June 28. An infection experiment upon larvae of 
Vanessa antiopa treated with spores from Nos. 26 and 27. About 
seventy-five nearly full-grown larvae collected from willow were placed 
in a fruit-jar, dusted with Sporotrichum spores, and then transferred 
to a wooden breeding-cage, the bottom of which was covered with 
a layer of damp earth and strewn with the food plant. Eight days 
later all the larvae had pupated. Most of the butterflies emerged 
by the 14th, but no fungus appeared. Two larvae, however, died 
in the check July 6, and one showed traces of Sporotrichum; other¬ 
wise the insects remained without loss. 
No. 42. August 15. A contagion experiment with chinch bugs 
exposed to infection from the Vanessa larva dead with Sporotrichum, 
taken from the check on the experiment next preceding. Several 
hundred chinch-bugs were placed in a small wooden box with 
green corn leaves for food, together with the larva dead with the 
white fungus. Several dead bugs were seen August 26, some of 
which were covered with Sporotrichum in a fruiting condition. 
Many other bugs were dead and imbedded in the same fungus 
by the 29th. 
No. 43. July 5. An unsuccessful infection experiment upon 
larvae of Papilio asterias treated with spores from cultures 26 
and 27. The larvae were thoroughly dusted with spores aud placed 
in a breeding-cage on damp sand with food plant. Part of the 
larvae died but no white fungus appeared in the cage. Many of 
these caterpillars died of flachene in both check and infected lot. 
No Sporotrichum seen at any time. Two butterflies appeared in 
the infected cage and two in the check. 
No. 44. July 15. An infection experiment with a lot of mis¬ 
cellaneous insects, mostly grasshoppers, dusted with spores of 
Sporotrichum from cultures 26 and 27. The insects were kept in 
a tight wooden box, dampened inside and containing a layer of 
dirt, with grass for food. The box was then placed on a bench 
in a cellar. The insects were all dead by August 1, but no fun¬ 
gus of the white muscardine appeared. 
No. 45. July 26. An unsuccessful experiment with army 
worms (larvae of Leucania unipuncta). Several hundred larvae 
were dusted with fungus spores from Nos. 26 and 27 and 
placed in a breeding-cage, with fresh oats and grass for food. 
The experiment was a total failure so far as the development of 
Sporotrichum wa^ concerned. The larvae had all died by August 
6; some with flacherie , and many others from attacks of parasitic 
Diptera and Hymenoptera. 
