77 
No. 46. August 8. An infection experiment on cabbage worms. 
Several larvae of Pier is rapce were treated with spores from Nos. 
26 and 27, and placed on a cabbage leaf under a bell-glass. All 
died from the attacks of Micrococcus and parasitic Diptera. No 
Sporotrichum appeared at any time. 
No. 47. August 8. A contagion experiment made with a lot of 
miscellaneous insects, mostly leaf-hoppers. The insects were placed 
in a wooden box 12x12x14 inches, and food supplied, together 
with small pieces (lees than half a dram in quantity) of the 
solid material bearing ripe spores from cultures 26 and 27. The 
box was then placed on the cellar floor. The insects were all 
dead by the 26th, and many of them bore a profuse external 
growth of Sporotrichum, which fruited abundantly a little later. 
No. 48. August 15. A contagion experiment with chinch-bugs. 
Several hundred chinch-bugs were placed in a small wooden 
box and supplied with corn leaves which had been previously 
dusted with fungus spores 'from 26 and 27. The box was then 
thoroughly moistened inside and placed on damp sand in a shady 
corner in the insectary. No evidence of disease was detected on 
the 18th, but three days later two bugs were dead, with an ex¬ 
ternal fungous growth. On the 26th many more were imbedded in 
Sporotrichum, much of it nearly ripe. August 29, still more 
were dead. No further record was kept. 
No. 49. March 14. Test-tube cultures on corn meal and beef 
broth infected with spores from cultures 7 and 14 of the series 
of 1891. Six tubes were prepared and treated with Sporotrichum 
spores. The fungous growth showed nicely four days later, 
and three tubes contained an apparently pure growth, while in 
three others the culture was mixed. Spores were forming on the 
22d, and apparently all were ripe on the 28th—fourteen days from 
the time ihe culture was begun. 
No. 50. April 11. A culture on corn meal saturated with beef 
broth infected with spores from No. 49. This culture was made 
with a view to finding a method of growing Sporotrichum in con¬ 
siderable quantity more conveniently than by the ordinary method 
already described. Two small cheese-cloth sacks were filled with 
corn meal and soaked in beef broth until the meal was saturated. 
They were then placed in fruit-jars and sterilized at 100° Cent, for 
one hour, and treated with Sporotrichum spores from No. 49. 
The fungus had made a short furry growth four days later, and 
on the 16th was quite vigorous on a part of the cloth surface. 
On the 18th almost the whole of the surface was covered with a 
good mycelial growth, spores had begun to form on the 19th, and 
a part of those were ripe by the 21st, or ten days after the be¬ 
ginning of the experiment. The growth on this cloth-covered sur¬ 
face was, however, much less profuse and fruitful than that on 
bare corn-meal batter. 
No. 51. May 9. Infection experiment with white grubs (Lach- 
nosterna) collected from ground in the orchard on the University 
farm, and treated with Sporotrichum spores from culture No. 49. 
The grubs were placed in a breeding cage similar to that used 
