78 
in No. 19. The experiment was fairly successful, and several dead 
grubs were removed whose bodies were covered with Sporotrichum, 
fruiting abundantly. Spores from two of these grubs were used 
to infect two agar cultures (Nos. 52 and 54), both of which were 
successful; and these were used, in turn, for inoculating other 
cultures. 
No. 52. July 8. Test-tube cultures on agar infected with 
Sporotrichum spores from a white grub removed from experiment 
No. 51. Mycelial growth had begun two days later. Slight traces 
of bacteria present on the 11th, the white fungus growing freely. 
Bacteria had disappeared the 14th, and the white muscardine was 
vigorous. Spore heads were forming the 15th, or seven days 
after the fungus spores were sown. 
No. 53. November 17. Cultures precisely similar to No. 52, 
and infected with spores from that experiment. Three tubes 
were prepared, in which the white fungus was growing fairly well 
three days later. It spread slowly, but in a pure culture, to the 
25th, and spores were abundant on the 30th, or thirteen days 
from the time the agar was infected. 
No. 54. July 15. Agar cultures precisely similar to No. 52, 
being infected with Sporotrichum spores from the same source 
(No. 51). Mycelial growth visible three days later, and spores 
ripe in seven days. 
No. 55. July 15. Six successful test-tube agar cultures infected 
with spores from culture No. 54. 
No. 56. October 31. A test tube agar culture infected with 
spores from No. 55. Slight mycelial growth in three days. Spores 
forming very abundantly November 7, and beginning to ripen on 
the 14th—fifteen days after the original spores were sown. 
No. 57. November 17. An agar culture, infected from the pre¬ 
ceding (No. 56) with similar results. 
No. 58. May *25. An infection experiment with wdiite grubs 
(Cyclocephala and Lachnosterna) dusted with spores of Isaria 
lejDvosa. This infection material came from an agar culture origi¬ 
nally made with spores from an earlier agar culture received from 
Dr. Thaxter March 21. The grubs were treated with spores, 
placed in a breeding-cage, and covered with blue-grass sod. They 
were left there undisturbed until June 29, at which time several 
dead grubs were removed and placed on damp sand The Isaria 
fungus developed rapidly on their bodies and was in a fruiting 
condition July 2. The check on this experiment was the same as 
that used in No. 21. 
No. 59. July 8. Test-tube cultures on agar treated with spores 
of Isaria leprosa from a grub out of No. 58. A good growth of 
mycelium had started July 10, which spread rapidly over the sur¬ 
face of the medium. Spores had begun to form in both tubes the 
16th, and were colored as in the original Thaxter tube the 20th. 
Spores fully ripe the 25th, and easily detached by shaking. 
No. 60. July 25. An infection experiment upon chinch-bugs 
treated with spores of Isaria leprosa from agar culture No. 59. 
