79 
The bugs were enclosed in a tight wooden box on damp sand,, 
and supplied with corn leaves for food. All had died by Septem¬ 
ber 5, and no traces of fungus of any kind were seen at any time. 
No. 61. December 13. Test-tube culture on agar infected with 
Isaria spores from culture 59. Results similar to those of No. 60. 
No. 62. July 7. Test-tube agar culture treated with spores of 
Isaria from the original Thaxter culture of Maicli 21. Pure white 
mycelial threads covered the surface of the agar by July 11, and 
spores were very abundant three days later. 
No. 63. October 31. Test tube agar culture infected with 
spores of Isaria from same source as No. 62. The fungus had 
started freely two day later; had begun to form spores and had 
assumed a distinct pinkish tinge November 12, and was fruiting 
abundantly November 14. Spores had the characteristic color of 
ripeness on the 19th 5 and were easily detached by shaking. 
No. 64. May 9. An experiment intended to test the possibility 
of a transfer of Botrytis tenella from one white grub to another 
in the earth. For this purpose thirty larvae of Lachnosterna were 
placed in a breeding-cage and covered with earth, and with these 
were buried, separately, five dead grubs covered with a dense 
growth of Botrytis,bearing spores, from experiment No. 12. Wheat 
was sown in the cage to fnrnish natural conditions and to afford 
food, and a check lot was established and similarly provided for. 
Seven days thereafter no effect was visible, but one grub was dead 
in the check. At the end of a fortnight two larvae had died in 
the experimental lot, but with no appearance of a fungus myce¬ 
lium. One month later (June 23) sixteen living larvae were fou ud 
in this cage, one had died from hymenopteroas parasitism, and 
the eleven remaining w 7 ere dead, but with no appearance of a fun¬ 
gous growth. Matters remained in substantially this condition 
until July 5, when one additional dead larva was found, together 
with two living pupae. August 26, when the experiment was 
abandoned, the cage contained eleven adult June beetles (Lachno¬ 
sterna), one remaining pupa, and a second larva killed by a 
hymenopterous parasite (Pelecinus polyturator). The dead white 
grubs whose remains were detected in the earth showed no trace 
of fungous infection, and the check cage w 7 as reported by the 
assistant who conducted the experiment, to be in practically 
parallel condition. 
No. 65.. April 19. An agar culture of Isaria leprosa , made in 
the botanical laboratory of the University with spores from the 
original Thaxter tube. A successful culture. 
No. 66. June 3. An agar culture like the four preceding, and 
infected with spores from No. 65. 
No. 67. November 3. Test-tube agar culture treated with 
Isaria spores from No. 66. A dense white mycelial growth cov¬ 
ered about half the nutrient material on the 7th, mycelial threads 
were of a pinkish tinge the 12th, and spores were forming the 
14th, the greater part of which were ripe three days later. The 
color had in the meantime deepened, and both mycelium and 
spores were fully colored November 19, where the agar was thin;. 
