81 
those used in No. 71. Many bugs were dying three days later, 
and by the ‘27th one was covered with mycelial threads, s< me of 
which were bearing spores. The death rate increased until July 
8, at which time all were dead, about half the insects placed in 
the cage being covered with Sporotrichum, as were several other 
species ( Anomcea laticlavia, Nodonota trishs , Formica fusca , and 
Chcetocnema subviridis), accidentally enclosed. One bug was 
found dead with the white fungus June 27 —almost certainly 
as a consequence of infection from the neighboring experimental 
lots. 
No. 75. May 6. An infection experiment with plant-lice treated 
with spores of Eniomophthora aphidis from chinch-bugs dead 
with that fungus, received from Dr. Snow in October, 1891. A 
geranium leaf covered with plant-lice, and a fungus covered bug, 
were placed in a bottle together and shaken up. Three days later 
the insects were transferred to a geranium on which lice were 
abundant. Several died a few days later, but no fungus appeared 
on any of them. 
No. 76. May 17. A test-tube agar culture infected with spores 
of Sporotrichum from an insect (Euschistus) collected May 10 in 
a wheat field near Tilden, Illinois. The fungus had begun to 
grow on the culture medium by the 20th; spores were forming 
three days later; were apparently ripe by the 30th; and were 
easily detached by shaking June 4. 
No. 77. November 17. A culture on agar infected with spores 
of Sporotrichum from No. 76. No Sporotrichum appeared, but a 
greenish mold grew instead, fruiting abundantly by Nov. 22. 
No. 78. May 25. A test-tube agar culture of Botrytis 
tenella, started with spores of a culture received from Dr. Thax- 
ter May 21. Thaxter’s material originally came from Prof. A. 
Giard, of France. A slight growth of mycelium had appeared in 
the tube by May 30, which mostly disappeared, however, by June 
6. Spores were forming on the fungus June 10, and by June 14 
were ripe enough to shake off. 
No. 79. July 7 Two agar cultures like No. 78, being infected 
with Botrytis spores from the same source. Four days later 
mycelial tufts were visible on the nutrient material, and the agar 
was slightly discolored, being of a brownish tinge. July 12 one 
tube contained a good growth of fungus, and the agar was slightly 
tinged with red. The growth in the other tube was not quite so 
vigorous. July 14 the growth was very strong, and the agar had 
turned a reddish tint like that in Nos. 4-10. By the 16th this 
reddish tinge was fading, and spore heads were forming on the 
surface. Mature spores were abundant by the 22d, and the red 
color in the agar had then almost disappeared. 
No. 80. June 28. A contagion experiment with caterpillars of 
Vanessa antiopa. Several Datana larvae dead w th Sporotrichum 
had been left in the breeding-cage described under No. 51, 1891. 
It must be borne in mind that these Datana larvae had been in 
this cage about eleven months. On June 28, 1892, they were well 
covered with spores whose vitality it was desired to test. Several 
—6 
