82 
larvae of Vanessa antiopa collected from willow were placed in the 
•cage. Five days later two had died and one had begun to pupate. 
By July 4, all of the caterpillars but three were attached to the 
top of the cage. Twenty-seven had died by the following day, and 
only nine had succeeded in pupating. An examination of one dead 
larva showed the presence of mycelial threads in its body cavity. 
By July 11 the white fungus had appeared externally on twenty- 
eight lame, and spores were forming on several. Only one insect 
succeeded in maturing, and this emerged July 14. 
No. 81. July 19. A farmer’s contagion box, started by Mr. 
Marten on the farm of Mr. F. O. Yantuyle, near Manchester, 
Illinois. The box w r as prepared as in No. 34, spores of Sporotri- 
chum being supplied from our laboratory. The box was examined 
every other day, and supplied with live insects and fresh food 
when needed. Abour. one w r eek later a quantity of bugs, both dead 
and alive, were removed and placed in two corn fields, behind leaf 
sheaths, where chinch-bugs were most numerous. The box, and 
the fields in which the diseased bugs had been distributed, were 
examined by Mr. Marten August 1*2. The box was in good con¬ 
dition, and contained mauy fungus-covered chinch-bugs. One 
chinch-bug dead with Sporotrichum was found under a clod in 
one of the fields where the fungus had been scattered, but aside 
from this no traces of muscardine disease could be found in the 
neighborhood at that time. , 
No. 8*2. July 20. Two test-tube agar cultures sown with Sporo¬ 
trichum spores from adult dead chinch-bugs taken from a barley 
field near Forreston, Illinois, June 20. Both cultures were suc¬ 
cessful, and produced good growths of the white fungus. 
No. 83. August 15. Au infection experiment with chinch-bugs 
collected at Forreston August 18. Several hundred bugs, pupae 
and adults, were placed in a test tube which contained Sporotri¬ 
chum in a frui ing condition, growing on agar agar, and shaken 
until thoroughly covered with spores. The iusects were then placed 
in two small wooden boxes and supplied with food, and the boxes 
were set on damp sand in the insectary. Several had died by 
August 21, but no external fungous growth was seen on their bodies. 
By the 26th, many in both boxes had died and were imbedded in 
fruiting Sporotrichum, and by the 29th, still others. With this the 
record ceases. 
No. 84. November 15. An agar culture sown with spores from 
a beetle dead with Sporotrichum, found near Urbana, Illinois, 
November 8. A few isolated spots of white mycelial threads 
were seen November 18. Bacteria also present in the tube 
at this time. A small tuft of this fungus transferred to a second 
tube of agar November 20. Growth very slow, but a dense mycelial 
cluster had formed by the 30th. Spores had begun to appear 
December 1, and the mycelial threads almost completely filled the 
lower part of the tube. Spores were abundant December 10, but 
had ripened very slowly. 
No. 85. April 19. Infection experiment on white grubs with 
-spores from agar culture made by Prof. Burrill. This culture was 
