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ripe spores of this fungus into their spiracles and over the inter¬ 
segment ai spaces. They were then placed under cover on damp 
sand in the insectary. April 10, apparently dead; although the 
bodies are flexible, they do not respond to irritation. April 14, 
bodies quite stiff. April 16, interior of bodies of these pupae 
filled with fungus mycelium. April 18, white mycelium growing 
from cuts in pupae. April 20, this mycelium growing in a thick 
tuft, and mere points of mycelium appearing also through the 
spiracles. April 23, mycelial tufts fruiting; others commencing to 
penetrate the chitinous pupal covering. April 27, spores devel¬ 
oped on the older mycelial tufts, and becoming faintly colored on 
the oldest. General penetration of the chitinous crust quite 
marked, especially beneath the thorax and the abdominal seg¬ 
ments. May 1, mycelium breaking through the surface every¬ 
where; spores ripe and fully colored on the older growths. May 
10, spores generally ripe. May 24, mycelium has penetrated a 
piece of thin card board on which the specimens were placed. 
No. 2. July 1. A general contagion box for the multiplication 
of Sporotrichum and the distribution of infected bugs to farmers. 
A quantity of chinch-bugs from Xenia, Illinois, inoculated July 20 
with spores of Sporotrichum globuliferum from a recent agar cul¬ 
ture, and placed in a cellar, in a wooden box, with grain and corn for 
food. July 22, additional chinch-bugs from Southern Illinois placed 
in the box; July 24, still others from Ashley and Xenia, in Southern 
Illinois; and July 26, again, from the southern part of the State. 
July 28, a few bugs dead with Sporotrichum. August 1, box thor¬ 
oughly overhauled; about one fifth of the bugs dead, a few of them 
covered with Sporotrichum and a few with Entomophthora aphid is. 
August 4, fresh food put in box. No noticeable increase of fungous 
infection. August 9, number of dead bugs not increased. August 
10, transferred to a new box, together with seven smaller collections 
(Nos. 3-9) received from various parts of the State, and which had 
been infected with artificial cultures of Sporotrichum July 28 
and 31. A large quantity of bugs collected from the field at Odin, 
Illinois, also placed in the box, most of them young. A quantity 
of dead bugs with external growth of Sporotrichum taken out and 
prepared for shipment. August 12, a few more dead, with fresh 
growth of fungus. August 17, Sporotrichum increasing very slowly. 
August 20, development of fungus still very slow. Box removed from 
cellar to shaded place on the sand floor of the insectary. August 22, 
several bugs noticed with fresh growth of Sporotrichum, and August 
24, still others. August 29 and 31, a gradual increase of fungus- 
covered bugs. September 5, box in excellent condition; many bugs 
dead with fungus, but at least as many without. September 6, box 
cleaned up, and enough dead and fungus-covered bugs taken out to 
supply eighteen correspondents. 
No. 3. July 28. Chinch-bugs dusted with spores of cultivated 
Sporotrichum, and placed with green corn in a wooden box. 
August 4, four bugs dead with surface fungous growth. August 
9, no more dead. August 10, transferred to No. 2. 
No. 4. July 28. Chinch-bugs dusted with spores of cultivated 
Sporotrichum and placed with green corn in a glass cylinder on 
