103 
Although several of the experiments here described, w ere incom¬ 
plete or otherwise unsatisfactory, I have thought it best to report the 
whole mass of them, as an assurance (if for no other reason) that 
nothing has been withheld because of its unsatisfactory character. 
No. 1. April 21. A test-tube culture on agar, from a dead in¬ 
sect larva of undetermined species, found in a field at Urbana, 
Illinois. April 24, had barely begun to grow. April 27, spores 
had formed. April 30, spores ripe, the culture unmistakably Sporo- 
trichum globuliferum. This larva and the culture derived froit 
are the starting point for all experiments of this list to No. 52, 
inclusive, as well as for Nos. 64, 65, 67, 71, 74 and 75 additional. 
No. 2. May 7, six Mason fruit-jars, with caps altered to facili¬ 
tate sterile culture (see Plate V., Pig. 1), partly filled with a bat¬ 
ter of corn meal and beef broth and sterilized by dry heat for one 
hour on each of two successive days, were inoculated by spores of 
the muscardine fungus taken from culture No. 1. This second 
culture was successful, and furnished material for a large amount 
of subsequent experimental work. 
No. 3. May 11, a lot of chinch-bugs received from F. O. Pierce, 
of Xenia, Clay county, was treated with spores from culture No. 1 
and placed in a small wooden box which was kept on wet earth 
under a hedge, and covered again with a box of larger size. May 
16 these bugs began to die. By the 22d all were dead but one, 
and an external growth of the Sporotrichum had begun to appear 
upon time. All were then returned to the sender for distribution 
in his fields. 
No. 4. A precisely similar experiment with chinch-bugs from 
Trenton, Illinois, was begun May 16, the experimental box being 
similarly placed. On the 19th some of the bugs were dead; on 
the 22d an external fungous growth appeared upon two of them, 
as yet, however, without spores; on the 25th many more were 
dead, several of them cohered with a white mycelium; and by the 
29th nearly all had perished, development of the characteristic 
spores on several of them now giving unmistakable evidence of 
the presence of & globuliferum -the special fungus of the mus¬ 
cardine disease. Specimens returned to the sender. 
No. 5. On the 17th of May a second lot of bugs from Xenia 
were similarly treated, with a similar result. Beginning to die 
May 19, most of the bugs were dead on the 22d, aud on the 29th 
all had perished. A slight external growth appearing at this date 
resembled in every way the immature mycelium of the muscardine 
fungus. Without waiting for further evidence of infection this lot 
of bugs was returned to the sender. 
No. 6. Next, on the 28th of June, nearly two months from the 
original agar culture (No. 1), a second agar tube was infected 
from that growth. July 6, this culture was ripe, and the spores 
were used for the experiment next succeeding. 
No. 7. July 6 a test-tube culture of corn meal and agar gela¬ 
tine was infected from culture No. 6—the third remove from the 
dead insect. July 9 this had made a good start, but was not fol¬ 
lowed further. 
