104 
No. 8. July 6. This was an experiment identical with No. 7, 
and Degun at the same time, varying only in the culture medium 
used, which was a batter of corn meal mixed widi water in which 
potatoes had been boiled. Three days later, July 9, the spores 
were starting abundantly, but the matter was not followed further. 
No. 9. July b. This was a companion experiment to the two 
preceding, except that our ordinary mixture of corn meal and beef 
broth was used in place of the foregoing media. July 9, growth 
had begun, but less vigorously than on either of the others. 
No. 10 July 9. A corn-meal batter, like that of No. 9, but 
acidulated with acetic acid, was infected with ripe spores from 
No. 1. July 11 it had made a good start, but was not followed 
further. 
Nos. 7, 8, and 9, derived from No. 6, were intended originally to 
test the comparative value of various corn-meal mixtures. The 
exigencies of the season’s work prevented their completion, and 
they serve merely to give some hints suggestive of further trials. 
■No* 11. July 2. This was a repetition of No. 6—a set of agar 
test-tube cultures, twelve in all, the spores for which were derived 
from No. 1. July 5 all were freely growing, and July 9 the 
giowth was spreading rapidly. These tubes were not again ro- 
poited on until August 13, at which time an abundant develop¬ 
ment of the muscardine fungus was noted on all, the gelatine 
having, however, in the meantime quite dried up. A long series 
of cultures on corn meal and of infection experiments on chinch- 
bugs and cabbage worms, and other larvae, were made with spores 
from this set of tubes. 
,N°- 12* # duly 13, 1:30 p. m., several fruit-jars of the corn-meal 
mixture with beef broth, neutralized with sodium carbonate, were 
infected with spores from No. 11. Two days later, July 15, these 
spores had begun to grow, and on the 17th the growth covered a 
part of the surface. 
No. 13. July 13, at 1:30 p. m., spores from No. 11 were sown 
on acidulated mixture of corn-meal and beef broth which had 
been placed in altered Mason fruit-jars and sterilized by heat as 
already described. On the 15th growth had begun, and on the 
1/th the surface of the meal was covered. 
No. 14. July 17, at 2 o’clock p. m., fourteen Mason fruit-jars 
of the acid mixture of corn meal and beef bioth, sterilized by 
heat on two successive days, were infected with spores from No. 
11. On the 19th the growth had started, and on the 21st it had 
become very abundant, distinctly more so indeed on both these 
acid mixtures (13 and 14) than on the neutral mixture, No. 12. 
No. 15. July 31. An infection experiment on living cabbage 
worms with spores from the acid corn-meal culture, NoM4 of this 
list. Two cabbage worms were infected on the back near the 
head, and were then shut up in a large covered glass dish which 
had a layer of moist sand on the bottom, on which a fresh cab¬ 
bage leaf was placed for food. August 1 no growth had appeared 
on either cabbage worm. August 2, still no growth, but one of 
the larvae had pupated. August 3, still no growth. August 7, 
