Next, I report five experiments with dead chinch bugs, intended 
to test the possibility of the infection of this insect after death 
by other causes. (See also Nos. 46 and 47.) 
No* 36. August 2. Fifteen chinch-bugs killed by crushing 
were placed in a covered glass dish, and treated with spores from 
cuiture No. 11. August 3, no appearance of growth. August 7, 
the dish a mass of mold, which fills the entire interior; no ap¬ 
pearance of a growth of Sporotrichum on the bugs. August 13, 
no development of Sporotrichum in this experiment. 
No. 37. August 2. A duplicate of No. 36, with precisely the same 
result. 
No. 38. August 2. Chinch-bugs killed with chloroform, placed 
on a cabbage leaf on moist sand in a covered glass dish and in¬ 
fected with Sporotrichum from No. 11. August 3, no growth. 
August 7, no appearance of infection; specimens beginning to 
mold. August 13, no Sporotrichum to be found. 
No. 39. August 2. Equivalent experiment, except that the bugs 
were placed immediately on sand. No growth August 3. August 
7, 3 p. m., slight appearance of Sporotrichum on legs, antennae, 
etc., of single insects, here and there. 
No. 40. August 2. The same as 38, August 3, no growth. 
August 7, loosely covered with bluish mould; no Sporotrichum 
formed. 
No. 41. August 2. Infection experiment with dead cabbage 
worms. Equivalent of No. 25. August 3, no growth. August 4, 
development of Sporotrichum commencing on all these cabbage 
worms. August 7, Sporotrichum well grown, with some appear¬ 
ance of post mortem mold. 
No. 42. July 3. A fiuit jar culture on corn-meal batter made 
with beef broth, the spores for which were taken directly from 
agar culture No. 1. A successful growth, not requiring detailed 
report. 
No. 43. July 3. An agar culture in five test-tubes, made, like 
42, from No. 1. Developed readily and matured in due seasoD, 
being subsequently used for a considerable number of cultures 
and infectious. 
No. 44. July 20. Fifteen jars of the acidulated corn-meal 
mixture (degree of acidity not given) infected with Sporotrichum 
from No. 43 at 1:30 p. m.. These cultures were all well started 
July 23, aud matured in due season, eight of the fifteen being, 
however, somewhat contaminated by Aspergillus. The contents of 
these jars were subsequently used in part for chinch bug infection 
experiments on the large scale. 
No. 45. July 20. Culture like 44, differing only by an abbre¬ 
viation of the sterilization process. Previous to this jars had 
first been sterilized by heat while empty, then stocked with the 
corn-meal mixture and sterilized again. In the present culture, 
jars and batter were sterilized together, once for all. Result the 
same as in 44. 
No. 46. July 20. Chinch-bugs killed by chloroform were 
placed dry in a test-tube, treated with Sporotrichum from 43, and left 
with the test-tube plugged with cotton. July 28, no fungous growth. 
