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No. 47. July 20. Like 46, except that the chinch-bugs were 
moistened with distilled water before being placed in the tube. 
July 28 and August 13, no growth at either date. 
July 27, a beginning was made with experiments intended to 
determine the temperature at which Sporotrichum would grow 
most freely. 
No. 48. July 27, 11 a. m. Aq agar test-tube sown with spores 
from No. 43 and kept iu laboratory at temperature of 80° Fall. 
July 28, 8:30 a. m., 74° Fah.; 11:30, 79° Fah. July 30, 8:15 a. 
m., 74 Fah., growth starting nicely. The average temperature 
by day in this culture was about 77°. 
No. 49. July 30. Auother tube, as above, placed in south 
window, temperature ranging from 77° to 95° Fah. July 30, 
growth well started. 
No. 50. July 27. Agar tube, as above, placed in incubator at 
97 Fah. at 11 a. m., and kept there at a constant temperature of 
100 Fah. July 30, growth not starting. August 13, these spores 
did not germinate. 
No. 51. July 27. An experiment with cabbage worms infected 
from No. 43. Several caterpillars ( Pieris rcipce) placed under a 
sterilized glass bell-jar with a large piece of fresh cabbage leaf 
lying on table without moist sand. Spores were shaken from 
agar culture and spread upon the worms with a sterilized platinum 
wire. July 30, several worms have died, but without appearance 
of fungous growth. One example, however, shows growth near 
the hinder end, and has turned black about one fourth of its 
length. July 31, the cabbage worm just mentioned has turned a 
crushed-raspberry color, and the Sporotrichum has begun to 
spread over the surface. Another larva has turned a similar 
color, and a Sporotrichum growth is appearing at one point. 
August 1, several of the worms have become crushed-strawberry 
color, but without visible growth. One has become brownish- 
green, and on this Sporotrichum filaments have appeared externally. 
August 7, everything dead but one imago recently emerged. Ten 
died in pupa stage; two larvae parasitized. August 13, very little 
external development of fungus has appeared in this lot of worms. 
No. 52. August 2. Large sphinx larva (Protoparce) chloro¬ 
formed, and placed in a large culture dish upon a cabbage leaf 
resting on moist sand. Spores from culture 43 sown upon the 
right side. August 3, no growth. August 7, mixture of fungi all 
over surface, but infected area with a conspicuous white patch. 
August 13, no Sporotrichum glohuliferum , only common mold. 
No. 53. This is a farmer’s contagion experiment made by Mr. 
C. S. Hollenbeck, near Tonti, Illinois, and not observed by us in 
the beginning. Infection material origiually obtained from my 
office September 7, 1893, and used to start contagion box, the 
contents of which were afterwards distributed in his fields with a 
result which he regarded as successful last fall. The box, with a 
considerable number of chinch-bugs remaining, was kept over 
winter in a warm and rather moist cellar, and about April 20 its 
contents were scattered in wheat in a joung orchard, care being 
taken to place the material where bugs were thickly congregated. 
