126 
on agar re-introduced. The garden soil was takeh from five to 
twelve inches below the surface, and was free from rubbish. Au¬ 
gust 2, all the live chinch-bugs in No. 70 placed in this box. 
August 4, large number of dead insects, but no fungus seen. 
Box in good condition. Fresh food introduced. August 6, many 
bugs dead with good growth of fungus and many others without 
it. Both Aspergillus and mites in small numbers on culture ma¬ 
terial. Food renewed. August 9, a large number of dead insects 
developing Sporotrichum freely. A very few mites and some As¬ 
pergillus found on the culture material, much of which was taken 
out of the box. Food renewed. Several anguillulids found on 
dead insects in the dirt. August 11, bugs dead with the white 
fungus on the increase. Aspergillus also present in considerable 
quantities. A few mites seen on culture material. Anguillulids 
very abundant, filling the body cavities of insects dead for a con¬ 
siderable length of time. Chinch-bug eggs very abundant on 
the culture material, some apparently about ready to hatch. Au¬ 
gust 15, condition of box about the same as when last examined. 
Sporotrichum still present in considerable quantity. August 18, 
amount of fungus about the same. Fresh food supplied, box 
thoroughly moistened. August 22, many adults and a few young 
dead with Sporotrichum, and several covered with Aspergillus. 
Still another greenish fungus was present on several bugs. Eggs 
hatching by thousands in this box. Mites increasing, and angud- 
lulids still present in small numbers. Fresh food supplied. Au¬ 
gust 25, a considerable number of insects dead with Sporotrichum. 
Aspergillus seen in small quantities. Live bugs, mostly adults, 
quite numerous. A few joung seen. Fresh food supplied. 
September 3. Sporotrichum still quite abundant. Several hun¬ 
dred whitened bodies picked from the surface of the earth and 
used for experimental purposes by Mr. Johnson at Edgewood and 
Odin, September 4 and 5 (see Nos. 66, 73, and -74). A few live 
bugs still present. Fresh food introduced. September 16, box 
overhauled and fresh food added. About as many fungus-covered 
bugs as on last date. About fifty dead with muscardine removed 
and used for field infection experiment at Odin September 19 
(see No. 73). Fresh food introduced. September 20, final over¬ 
hauling. Several hundred whitened bodies picked out, which were 
distributed by Mr. Johnson to farmers in the south-central part 
of the State early in October. Only an occasional live insect 
seen. Box discontinued. 
No. 69. June 23, a second contagion box, precisely similar to 
No. 68, stocked with a large quantity of Jive chinch-bugs received 
from correspondents. The infection introduced was from No. 54. 
Examined June 26; picked out a few fungus-covered bugs and 
added fresh food and chinch-bugs. June 27, all the material, dirt, 
bugs, and food removed and scattered in wheat (61), except a few 
whitened bodies reserved for re-stocking the box. Box thoroughly 
cleaned. June 28, fresh food supplied and a considerable number 
of live bugs introduced with the fungus-covered bugs removed 
yesterday. June 29, fresh earth introduced, and cultivated spores 
on agar, from No. 11, added. 
