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the fourteen.hour lot revived, and were active in twenty to forty 
minutes; four from the sixteen-hour lot, and nine from the 
eighteen-hour lot were dead when removed, all the others reviving 
and crawling feebly about in twenty to forty minutes; and four¬ 
teen from the twenty-hour lot were dead when removed, the others 
barely showing signs of life but not trying to escape. A single 
bug in the twenty-two hour lot survived the immersion; but all of 
those in the twenty-four and twenty-six hour lots were dead when 
removed. From these facts it appears that adult chinch-bugs of 
the first generation cannot withstand complete immersion longer 
than twelve to twenty-two hours, the latter time being about the 
limit. The bugs which had been immersed ten hours were placed 
in a four-ounce bottle without food and corked up. Two dead 
bugs were seen August 25, and several eggs were noticed on the 
sides of the bottle August 27. They had been deposited several 
days before, for the young bugs were fairly well matured within. 
These eggs batched September 7, and the young bugs lived in 
this prison-cell for two days. Six more adult bugs were found 
dead on this date, and all except one had died by the 10th. The 
last one, a female, died September 12. This is simply given as 
an example of the wonderful power of endurance these insects 
possess. From the above it is clear that these bugs lived in these 
bottles without food and practically without air from fourteen to 
thirty-one days after having revived from a ten-hour complete im¬ 
mersion in water; and that in the meantime they paired and pro¬ 
duced eggs from which young emerged that lived two days. 
No. 6. On account of pressure of other experimental work these 
experiments were not repeated with insects of all stages of the 
second generation, but a long series of immersions was begun 
September 12, with adults of this generation. The insects were 
selected with the same care as in the first series, and twelve lots 
were immersed in a similar way. Beginning with an immersion 
of twenty-one hours for the first lot, three hours were added for 
the second, and the time was lengthened at this rate for each 
successive lot, bringing it up to fifty-four hours for the twelfth, 
when the experiment was permanently interrupted by more im¬ 
portant work. I give herewith in detail the results reached with 
these adults of the second generation: All the bugs in the twenty- 
one, twenty-four, and twenty-seven-hour lots revived in from twenty 
to forty minutes and were very active; in the thirty-hour-Jot four 
bugs did not revive, while the others seemed to be in good con¬ 
dition one hour later; in the thirty-three, thirty-six, and thirty- 
nine-hour lots the dead insects numbered respectively seven, twelve, 
and thirteen, while the others seemed to be in good condition and 
were actively moving about in the bottle an hour later; in the 
forty-two and forty-five-hour lots twelve and fourteen insects died 
respectively, while the other bugs revived in an hour; in the forty- 
eight and fifty-one-hour lots the numbers dying were respectively 
fourteen and fifteen, the other insects reviving in about half an 
hour and crawling about the bottle; and in the fifty-four-hour lot 
only three bugs survived the treatment, but these were lively and 
making attempts to escape at the expiration of that time. 
