W. L. Yakimoff and others 
75 
weight of the mouse, and the therapeutic dose is 0-0008 (in solution 1 : 2000), 
the ratio y c 0-0008 1 
dt ~ 0-0011 7 1*37 ’ 
Therefore, the therapeutic coefficient is not favourable. 
The action of the preparation on trypanosomes is very energetic. 
We have observed the disappearance of trypanosomes in one mouse. 
Mouse No. 440—2. iv. 1915, number of trypanosomes + + + +; 1 c.c. of 
a 1 : 1200 solution of tartar emetic was injected intravenously. 
5 minutes later the number of trypanosomes = 
10 
99 
99 
99 
zzz 
15 
99 
99 
99 
Z=2 
20 
97 
99 
99 
= 
25 
99 
99 
99 
= 
30 
>9 
9 
99 
= 
35 
99 
99 
99 \ 
40 
99 
i9 
’> 
1 
50 
•t j 
•> 
60 
99 
99 
} = 
70 
99 
99 
*9 
80 
99 
99 
99 
90 
99 
99 
,, > 
100 
99 
99 
99 
= 
+ + + 
+ + 
+ + 
+ 
+ few 
+ few 
+ very few 
0 
Experiments with Trypan-blue. This preparation was originally introduced 
in the therapy of experimental trypanosomic diseases by Mesnil and M. 
Nicolle. In the practical treatment of trypanosomiases it has not been applied, 
but is very important in the treatment of piroplasmoses as first demonstrated 
by Nuttall and Hadwen. 
The toxicity of the preparation at our disposal is shown in Table 17. 
Thus, the dosis tolerata is 1 c.c. of the solution 1 : 300. 
Table 17. 
Solution 
Results 
1:200 
Succumbed 
l : 300 'j 
1:400 \ 
Recovered 
o 
o 
ic 
r—H 
The effect of trypan-blue on the trypanosomes is shown in Table 18. 
Table 18. 
Days 
Solution 
i 
2 
3 
4 
5 
6 
7 
— \ 
8 
l : 300 
+ +© 
+ + + 
+ + + + 
+ + + 
+ + + + 
death 
o 
o 
+ v.f. © 
+ 
+ + 
+ + + 
+ + +' + 
+ + + + 
+ + + + 
death 
l : 500 
+ + © 
+ + + 
+ + + 
+ + + 
death 
l: 600 
+ V.f.© 
+ f. 
+ + 
+ 
+ + 
+ + + 
+ + + 
death 
l : 800 
+ v.f.© 
+ 
+ 
+ + 
death 
l : iooo 
+ v.f.© 
+ 
+ + 
+ + + 
99 
l : 1500 
+© 
+ + 
+ + + 
+ + + . 
99 
l : 2000 
+ f.© 
+ + 
+ + 
+ + + 
99 
