P.-W. Bassett-Smtth 
199 
that of acute icterohaemorrhagica. No. 4 guinea-pig, inoculated with heart- 
blood; this died on the 7th day showing the usual acute symptoms with 
abundant spirochaetes in the blood and in the haemorrhagic urine. The heart- 
blood was inoculated into a fifth guinea-pig. On the 4th and 5th day about 
1 c.c. of serum from guinea-pig No. 2 a was given, but this either contained 
no immune body or insufficient, as the animal died on the 6th day. A sixth 
guinea-pig was inoculated on the 21st May from the heart-blood of No. 5, 
containing spirochaetes; on the 23rd, 24th, 25th, 27th, 29th, and 4th June, 
1 c.c. of the Pasteur Institute curative serum was given subcutaneously. The 
animal never showed any signs of the disease, and was killed on the 51st day. 
At the autopsy all the organisms were healthy and no spirochaetes could be 
found, also its blood and urine were not infected by inoculation. 
A white rat inoculated with infective blood was immune. 
CULTURE or SPIE.OCHAETA ICTEBO-HAEMOBBHAGICA 
FROM PASTEUR INSTITUTE, MAgCh 2.1 1^18 
GPN?I SUBCUTANEOUS INOCULATION. HAPCM 27 l<^8 
result 
KIU.C.0 l-a'DAT_APRIL 20K)i» MO SPIROCHAETES FOUND ANIHAL QUITE WELL 
I- 
UBINE 
DiEO h-dat_G.P N ? 2 
WILED r’DAY_I_ 
-1 
8 L 00 D (h*A»T^ 
GP N?2A 
MO SPIROCHAETES found 
. _ ANIMAL QUITE WELL 
UfclRtBLoio (HEART*, 
CMt.o(aoTM| -i*oat_GP N*3_GPN ? SA 
otto voay _GP N® 4 
DIED A"DAY _GP N°5 
GPN°G. 
TREATED WITH SIX D05ESOF 
. SCRUM ; NO SlSN OF SPIROCHAETOSlS 
KILLED 51“ DAY 
The experiment shows: 
(1) The primary culture, though living when used for inoculation at 
Greenwich, had lost much of its toxicity, and though the animal when killed 
on the 24th day gave no microscopical or cultural evidence of spirochaetes, 
yet the urine by inoculation was lethal in nine days, giving rise to typical 
symptoms. The local infection had remained but the septicaemia had passed 
off, for the serum of an animal inoculated with the blood contained no pro¬ 
tective antibodies. 
(2) By passage the toxicity was gradually increased, killing on the fifth 
passage on the sixth day. 
