F. W. O’Connor 
251 
Throughout the treatment and for three weeks afterwards, during whicli 
patient was carefully observed, there was no change in the physical condition 
or in the number of oocysts passed. 
Case R. This patient was the one in whose stools the oocysts were found in 
the Entamoeba coli. Although he passed even more oocysts than Case S. yet 
he complained of no symptoms which could be attributed to the parasite. 
1 therefore have no conclusive evidence to prove that Isospora is or is not 
pathogenic. 
Animal Experiments with Isospora. 
An attempt was made to infect two new-born puppies with Isospora 
from Case S. The puppies were placed within a few hours after birth in a 
fly-proof cage about ten feet above the ground and they were milk-fed through 
a pipette. When one month old Puppy A was given two teaspoonfuls of well 
macerated faeces in which there were numerous fully developed oocysts. 
Milk was used as a medium. The stools, which were normal, were frequently 
examined and no parasites were found. The puppy remained well for three 
weeks. On the 21st day after the administration of the dose, evidence of 
weakness of the hind legs was noticeable, and towards the end of the same 
day efforts to maintain the erect position completely failed. The abdomen 
was very much distended and respiratory distress became marked. Next 
morning as the distress was very great the animal was chloroformed. Some 
liquid motions had been passed for two days before death; cells were found in 
these, but none resembling the schizonts or oocysts of Coccidia. On post 
mortem examination the large and small intestines were found distended with 
flatus. The first two inches of the small gut were markedly hyperaemic and 
there was a quantity of mucus in this part of the bowel; a few hyperaemic 
patches were also seen in the caecum; the glands in the omentum were 
swollen and soft; the liver, spleen and lungs showed no evidence of disease. 
Microscopical examination of the mucus and of the intestine and liver, fixed 
at the time of the autopsy and subsequently stained, showed no evidence of 
coccidial infection. Haemorrhages were found in the submucosa of the intes¬ 
tine and extending up the centre of the intestinal villi. The glands were 
found to have undergone advanced fatty degeneration. 
Puppy B infected a week after Puppy A died after 23 days under exactly 
similar circumstances. Post mortem examination showed the same changes 
as in Puppy A. No evidence of coccidial infection was found in either case. 
Vegetable Cells found associated with Diarrhoea. 
Whenever vegetable cells were found apparently causing diarrhoea, the 
appearance of the stools and the symptoms complained of were fairly constant. 
The cells found were: 
1. Blastocysts in various forms. 
2. Vegetable organisms resembling Chilornastix cysts. 
3. “1 cvsts.” 
