82 
“June 9, prepared beef broth by the usual method for pure 
bacterial culture, sterilized it, and" heated corn meal almost to 
scorching. Mixed the two to a thick batter, and put into a test 
tube, inclined to give a sloping surface. Plugged with cotton 
and sterilized at temperature of 100° Cent, for half an hour. 
Sporotrichum spores from an agar culture sown on this mixture 
June 11, 8 o’clock a. m. June 13, 8 o’clock a. m., growth of 
the fungus has begun. June 14, growing freely. June 15, spread¬ 
ing very rapidly. June 16, growth spreading freely and entering 
little crevices at the margins of the mass. Heads of spores be¬ 
ginning to form. June 17, fungus covering all the exposed sur¬ 
face. June 19, surface slightly tinged with yellow, indicating 
ripening of spores. June 22, masses of spores may be detached 
by vigorous shaking. June 24, spores readily detached; surface 
decidedly brownish. 
“June 12, materials prepared as above. From a lot of chinch 
bugs obtained in the field June 2, in which the Sporotrichum 
had appeared spontaneously, a dead specimen was taken June 
12, 3 p. m., and dropped into the tube on the surface of the 
meal. June 14, growth beginning. June 15, fungus growing 
freely all along the track touched by the chinch bug. June 17, 
surface of the meal well covered with growth; blue mould ap¬ 
pearing at the bottom of the tube. June 18, Sporotrichum 
as before; blue mould spreading. June 20, spore clusters of 
Sporotrichum showing quite distinctly. June 22, spores quite 
yellowish and loosened by jarring. June 27, a peculiar pink 
color apparent along the margins of the mould, penetrating 
deeply into the meal.” On examination this color proved to be 
due to a bacterial species which was transferred to an agar tube 
for separate cultivation.” 
Numerous attempts were made, beginning June 15, for the 
artificial culture of the Sporotrichum in the open air, without 
resort to the sterilization methods of the experiments given 
above, but all were unsuccessful. The materials used were corn 
meal saturated with beef broth; the same acidulated with acetic 
or tartaric acid for the purpose of retarding the development 
of bacteria; corn meal soaked with a modified form of Cohn’s 
solution, acidulated as before; corn meal mixed with water: 
corn meal soaked with an extract of the excrement of horses, 
variously acidulated with tartaric acid; the solid excrement 
itself; the tliitk scum from beef broth; and raw beef alone. 
Infection experiments were made with chinch bugs, Cecropia 
moths, the grain aphis and other plant lice and with saw-fij 
larvae (from grass). 
The first chinch bug experiment was begun May 11, 1891 
with about fifty specimens placed under a bell glass with frest 
wheat, and exposed to infection from several dead beetles ( Dis 
onycha pennsylvanica) covered with fungus spores from a lot o 
that species which had largely perished with this fungus attack 
