83 
?hree days later one chinch bug’ was dead, and in five days an¬ 
ther also. May 19 two more, covered with Sporotrichnm, 
/ere taken from the cage. May 20, at 8 a. m., another, 
rhich had certainly been alive at 2 p. m. of the preceding day, 
/as removed, whitened with the mycelium of the fungus. On 
he 22d of May two more had died from the fungus attack, 
nd on the 23d another. By the 29th of the month, eighteen 
lays after the experiment began, all but one of the chinch bugs 
/ere dead and covered with the Sporotrichum, and this one died 
hortly after. Other specimens of the same lot, kept in similar 
onditions as a check, had in the meantime continued without 
OSS. 
Next, May 29, thirty insects were placed, with a wheat plant 
or food, in a breeding cage, and two dead specimens whitened 
vith the fungus growth were placed among them. Twenty- 
our hours later these dead insects were removed. On the 3d of 
une, five days from the beginning of the experiment, one in- 
ect was dead with the fungus affection, and on the 7th two 
core. Three days later two additional specimens were removed 
rom the cage, whitened with an abundant growth of Sporotri¬ 
hum, and the remainder of the chinch bugs in this lot were 
hen converted to another use. None had died in the meantime 
n the check lot. 
June 1st a similar experiment was begun with thirty chinch 
mgs, among which four infested ones were introduced and left 
wo days. By June 10 three had died with the fungus attack, 
nd the remainder were then otherwise used, the check lot hav- 
ng in the meantime remained without loss. 
June 5 thirty chinch bugs were dusted with spores from an 
,gar culture of Sporotrichum, and two days later two of these 
/ere dead. June 9, two others had died, and by the 11th nine 
>f the thirty had perished, with a rapid subsequent develop¬ 
ment of Sporotrichum from their bodies. Unfortunately, howe¬ 
ver, for the significance of these results, six specimens from the 
heck of thirty originally separated had perished in the same 
nanner, thus giving evidence either that the original lot (ob- 
ained from Litchfield, Montgomery county) had been diseased 
/hen taken, or else that the check had become infected from the 
neighboring experimental lots. 
.Similar experiments on other insects were tried with similar 
esults. Saw-fly larvae (Tenthredinidae), obtained by sweeping 
rom grass May 22, were dusted with spores from the Thaxter 
ulture and placed under a bell glass on growing blue grass and 
lover. On the 26th two larvae were dead and covered with the 
tmgus, together with a small fly (Dolichopodidae) accidentally 
nclosed with them. May 28 more larvae were affected, and by 
he 30th all were dead. In the same lot were larvae of Apatura 
.nd several* plant lice, all of which died in the same manner. 
>aw-fly larvae enclosed without treatment had all survived. 
