134 
mycelium was first shown April 16, on a grub which had died 
on the 8th and had then been transferred to damp sand and 
covered with a bell jar. Others showed this external growth on 
the 18th and 20th, while spores first appeared April 26. From 
such spores successful cultures were made in test tubes of agar- 
agar, the culture medium assuming the deep red color to which 
this fungus gives rise. Proof was consequently complete of the 
destruction of at least a considerable number of these grubs by 
a thorough infection with spores of Botrytis tenella. In the 
check lot, in the mean time, three larvae had died; one on the 
14th and two on the 18th of April—all of them, however, with¬ 
out any appearance of fungous affection. 
A single experiment was begun May 9,1892, intended to test the 
possibility of the transfer of the disease characterized by the fun¬ 
gus Botrytis tenella from one white grub to another in the earth. 
For this purpose thirty Lachnosterna larvae were placed in a 
breeding cage and covered with earth, and with these were buried 
separately five dead grubs covered with a dense growth of the 
spore-bearing mycelium of Botrytis from one of the experiments 
just described. Wheat was sown in the cage to furnish natural 
conditions and to afford food, and a check lot was established, 
similarly provided for. Seven days thereafter no effect was vis¬ 
ible, and one grub was dead in the check. At the end of a fort¬ 
night two larvae had died in the experimental lot, but with no 
appearance of a fungus mycelium.' One month later (June 
23) sixteen living larvae were found in this cage, one had died 
from hymenopterous parasitism, and the eleven remaining were 
dead, but with no appearance of a fungus growth. Matters 
remained in substantially this condition until July 5, when one 
additional dead larva was found, together with two living pupae. 
August 26, when the experiment was abandoned, the cage con¬ 
tained eleven adult June beetles (Lachnosterna), one remaining 
pupa, and a second larva killed by a hymenopterous parasite 
(Peleeinus polyturator) . The dead white grubs whose remains 
were detected in the earth showed no trace of fungous infection, 
and the check cage was reported, by the assistant who performed 
the experiment, in practically parallel condition. 
May 25, 1892, a third experiment was begun with this fungus. 
Fifty grubs, chiefly Cyclocephala, with a few Lachnosternas, were 
thoroughly dusted with spores of Botrytis tenella\ and placed in 
a cage with blue-grass sod, a check lot being established at the 
same time. June 29, this cage was overhauled, and ten live 
grubs, three dead ones, two pupae and eleven adult Cyclocephalas- 
were found. One of the dead larvae had developed white myce¬ 
lium; another was characteristically pink in’color; while the 
