135 
th^ showed no sign of disease. The dead larvae were placed 
on damp sand, and by July 8 one had formed spores of 1 otry- 
tis from which successful cultures of tenella were afterwards ob¬ 
tained. The check lot developed fewer imagos, and contained a 
dead larva and pupa on June 29. These failed to develop para¬ 
sitic fungi when placed on damp sand, and this experiment was 
not carried farther. 
Perhaps the most successful attempt to kill our common white 
p;rubs with this Botrytis in our breeding cages began August 26, 
1892 when nineteen grubs from a corn field were thoroughly 
covered with spores from a culture made in agar-agar m July. 
The dusted grubs were placed in a breeding cage, without food, 
and moistened from time to time with water. September 24 ten 
of them were dead, and spores had formed on four W ltlim the 
bodies of the others a mycelial growth was discernible, but none 
appeared on the outer surface. The cage was not again dis¬ 
turbed until March 28, 1893, when eight more dead grubs were 
found only two of them showing fungus spores. 1 he six others 
contained no mycelium, and probably died from other causes. 
From the foregoing it appears that no clearly distinguishable 
effect was produced by this attempt at artificial infection. 
Similar experiments with this same fungus species were made 
in 1892 by Prof. Herbert Osborn, of Ames, Iowa, and by Mr. 
F W. Mally, at Washington, both under direction of L)r. liiley, 
Entomologist to the U. S. Department of Agriculture. 
Bv Prof. Osborn, forty-three Lachnosterna larvae were used in 
two^experiments and a number not given in a third with the 
effect to convey the fungus only to a single grub; and here, as 
no culture experiments are given, it seems possible that some 
other fungus may have appeared. 
In Mr Mallv’s experiment nine grubs were used in two experi¬ 
ments, and eighty-eight others were kept as checks. Here also 
the treatment was without result. 
The material used by Osborn and Mally was apparently de¬ 
rived from a commercial source (Fribourg & Hesse, Lb rue des 
Ecoles, Paris), and may have been in a condition inferior to 
that from Prof. Giard, with which my own experiments were 
m ade 
Experiments with Isaria leprosa, Er., were begun April 19, 
1892, in accordance with a suggestion made by Dr. Koland 
Thaxter, from whom a culture tube erf this fungus was received 
March 21 This culture was repeated on agar by 1 rotessor 
* Rep. U. S. Dept. Agr., 1892, p. 1G3. 
