136 
Burrill of the University of Illinois, and April 19 twenty ^vbs 
were dusted with spores from this second growth, and" placed 
in an earthen vessel which was filled with leaf mould and sunken 
m the earth. Grubs died in this lot April 23, 25, and 27, most 
of them becoming firm to the touch and of a duskv brownish 
hue. 1 he record shows the death of thirteen, in all," of this ex¬ 
perimental lot up to May 20, at which time ants invaded the 
cage, and the experiment was discontinued. Five dead speci¬ 
mens transferred to damp sand May 30, simply decayed with¬ 
out visible fungus growth. Only two of the lot, in fact, formed 
a mycelium, and none matured spores. 
The second lot of larvae, thirty in number, Cyclocephala and 
Lach nostern a mixed, was dusted Mav 25, 1892, with spores of 
Jsaria leprosa , from the above-mentioned culture on ao-ar 
they were placed in a breeding cage and left undisturbed untii 
June -9, when five dead larvae, fifteen living ones, two pupae 
and one adult 0\clocephala were found, the remaining seven 
haying died and disappeared. Two of those taken dead from 
the cage were placed on damp sand June 29, and grew the fun¬ 
gus iapidh in the form of stalk-like bundles which bore spores 
abundantly by July 1. Agar cultures made from these spores 
on the 8th of July were indistinguishable from the orignal cul¬ 
ture or from the fungus growing on the grubs. Theie was no 
appearance of a fungus growth on any of the grubs in the check 
lot corresponding. 
The common parasitic fungus Sporotrichium globuliferum 
characteiistic of the so-called white muscardine of the chinch 
bug, was also used by us at this time in these w T hite grub ex¬ 
periments, and seemed on the whole more efficient than either 
Botrytis tenella -or Isciria loprosci. April 19, 1892, thirty °rubs 
taken from an old corn field w 7 ere thoroughly covered^with 
spoi es of Spoi otrichum from a culture made on corn meal wet 
with beef broth. This culture was derived directlv from dead 
insects which had been collected at Urbana February 23. The 
specimens were moistened and then rolled about in the funo* U s 
spores until they were almost completely covered, looking as if 
they had been rolled in flour. They were buried in leaf mould in a 
crock, which w r as sunk in the earth in the insectary, and kept 
covered with a board and a wet cloth. The first result appeared 
April 2o, when one grub w T as dead and tw T o more were scarcely 
alive. ^Others were found dead the 27th and 30th, May 2, 7, 
and lo, and June 1 and 15—twenty nine in all. An external 
mycelium appeared first April 30, and by May 7 15 it was present 
on twenty-two of the grubs, and by June 15 on all but four of 
the lot. Spores wnre first formed (on two specimens) May 7 3, 
and by June 1 on several others also. 
A second lot of thirty grubs was dusted with spores of Bpo- 
rotrichum glohulileruin May 9, 1892, from the same culture as 
that used in the above experiment. May 16 nine grubs were 
dead, and by June 23 only seven remained alive. Later, three 
of these d:ed and four became adults. Dead examples transferred 
