80 
Insect Flagellates , etc. 
a footing there, being nearly always present either free or in leucocytes in the 
blood, peritoneal exudate and organs. These experiments have, however, again 
shown the extent to which the insect flagellates are capable of retaining their 
vitality in the body of the fish under conditions unusual to them. It is seen that 
they can be found alive in the peritoneal cavity and subcutaneous fluid of 
the fish for two to four days, but they disappear altogether on the sixth day, 
being probably phagocytised, as on several occasions I was able to find 
leucocytes that contained flagellates in degenerate condition within them. 
A summary of these experiments is given in Table III. 
Table III. 
Experiments on inoculation of sticklebacks with H. jaculum. 
Interval be- 
No. of 
experi¬ 
ment 
Mode of 
inocu¬ 
lation 
Date of 
inocu¬ 
lation 
tween inocu¬ 
lation and 
examination 
Results of examination 
of fresh blood and body 
fluids 
Results of examination 
of stained smears 
Remarks 
27 
i.p. 
28 x. 20 
24 hrs 
(died) 
In perit. fluid motile 
flagellates and bacteria. 
Blood—negative 
Blood, liver and 
spleen—negative 
Death due to mechanical 
injuries 
28 
2 days 
(killed) 
In perit. fluid motile 
flagellates. Blood— 
negative 
Blood, liver and 
spleen—negative. 
Liver contained 
bacteria 
29 
4 days 
(killed) 
In perit. fluid single 
flagellates, numerous 
bacteria. Blood— 
negative 
Blood, liver and 
spl een—negative. 
Perit. fluid con¬ 
tained numerous 
leucocytes 
Fish looked weak. Post 
mortem genitalia 
showed signs of hae¬ 
morrhage. (Mechanical 
injury) 
30 
— 
— 
6 days 
(killed) 
In perit. fluid no flagel¬ 
lates, numerous bac¬ 
teria. Blood—neg. 
Negative 
Fish quite normal 
31 
s.c. 
26 x. 20 
30 min. 
(died) 
Subcutaneous fluid con¬ 
tained motile flagellates 
Died from penetration 
of air introduced by 
injection 
32 
— 
— 
24 hrs 
(killed) 
In subcutaneous fluid no 
flagellates; numerous 
leucocytes and bacteria 
Negative. Spleen 
and Liver con¬ 
tained bacteria 
Slight swelling on one 
side of the body 
33 
2 days 
(killed) 
In subcut. fluid no flagel¬ 
lates. In perit. fluid 
several flagellates free 
and phagocytized, and 
bacteria 
Negative. Liver 
contained bac¬ 
teria 
34 
Remained quite norma 
for 3 weeks, when if 
was transferred to a 
tank with other fish 
c. 
Experiments with Amphibians. 
I. Newts (Molge vulgaris) inoculated with , and fed on, H. jaculum. 
(a) Newts inoculated intraperitoneally. November 11th. 
Experiment 35. Newt killed after 24 hours. No forms of the flagellates found in fresh 
peritoneal fluid and blood, nor in stained smears of the same, and liver and spleen. 
Experiment 36. Newt killed after two days. Results as above. Peritoneal fluid contained 
numerous leucocytes. 
