Fig. 4. Smear, fixed and stained, showing the spores with central cells and spirals. 
Fig. 5. Transverse section of Dasyhelea larva very heavily infected with Helicosporidium. 
Fig. 6. Transverse section of Dasyhelea larva not heavily infected with Helicosporidium. 
Fig. 7. Spores and unrolled filaments of Helicosporidium in fixed and stained smears. 
Fig. 8. Dead body of Dasyhelea larva infected with Helicosporidium, the spores of which are open 
and the spiral filaments free and unrolled. The body of the larva is ruptured by the 
pressure of a coverslip and shows an enormous number of escaping filaments. 
Fig. 9. A portion of the same mass of filaments under a higher magnification. 
PLATE VI. 
Life-cycle cf Helicosporidium parasiticum. 
Figs. 1-9. Different phases of schizogonic multiplication, formation of four- and eight-celled 
schizonts. 
Figs. 10-13. Formation of four-celled morula which develops into the spores. 
Fig. 11. Hypothetical stage showing the three central discoidal cells surrounded by the fourth 
cell. 
Fig, 15. Young spore before the formation of spiral filament: (a) side view, (6) end view. 
Fig. 16. Mature spore: (a) side view, ( b ) end view. 
Figs. 17-20. Different stages of the opening of the sporocyst, unrolling of the spiral filament and 
liberation of sporozoites. 
Stages 1-16 occur in the living larva of Dasyhelea, while the stages 17-20 are found only in the 
dead body of the host. 
Parasitology xm 
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