270 
Intestinal Protozoa in Termites 
distinct tubular organ of uniform thickness, measuring some 1 /a in breadth 
and 4-7 /x in length. It is clearly visible in living specimens as a transparent 
and refractive rod. It takes iron-haematoxylin intensely, its wall appearing 
as though consisting of closely packed granules. In the majority of cases, its 
posterior end is directly connected with the nuclear wall, and it is at their 
point of contact that the chromatic bodies are attached. In many cases 
distinct granules can be recognized at the two points of connexion of the 
chromatic bodies and the nuclear wall. The tubular structure is neither 
closed nor provided with a special structure at the anterior end; and it is 
from the edge of its circular anterior opening that the characteristic bands of 
deeply stainable granules lying under the periplast originate (PL XIII, fig. 35). 
The substance forming the contents of the tubule is dense and homogeneous, 
and takes eosin somewhat intensely. As described above, the nucleus is 
surrounded by a layer of dense protoplasm, and this layer is especially con¬ 
spicuous in the front part of the nucleus; that is, round the posterior part of 
the tubule. In large-sized forms the tubule is frequently detached from the 
nucleus, or its hinder portion is indistinct, and it is apparently kept in con¬ 
nexion with the nucleus by means of the particularly dense surrounding mass 
of protoplasm (PL XIII, figs. 36 and 37). 
Dividing forms are met with very rarely in this species, and despite my 
eager search, only a few forms in later stages of the process were observed. 
In PL XIII, fig. 38, is shown an individual in a stage just before the completion 
of division. There we see a filamentous structure, its central portion forming 
a distinct filament, and its ends consisting of deeply stained granules arranged 
in a chain. Two distinct chromosomes are found symmetrically situated near 
the ends of the filamentous portion, embedded in a mass of homogeneous 
protoplasm. Unfortunately I have been unable to find sufficient material to 
ascertain the origin of these structures. It cannot be doubted, however, that 
the process of division is analogous in essential points to that of Trichonympha. 
Further discussion of these points will be given in Part II. 
V. Holomastigotoides. 
This is the genus proposed by Grassi (1911) for an organism found in the 
Brazilian species of Coptotermes, and formerly distinguished by Hartmann 
(1910) as the “female” or “Form B” of “ Trichonympha hertwigi As already 
remarked, there are sufficient reasons to disprove Hartmann’s conception 
regarding the genetic relationship between his “male” and “female” forms, 
and Grassi’s action in establishing the genus was undoubtedly right. As 
regards the affinity of his “female form” with other forms, Hartmann re¬ 
marked: “sollte sie sich bei weiteren Untersuchungen wider Erwarten doch 
als eine besondere Art herausstellen, so miisste sie wohl in der Gattung 
Dinenympha gestellt werden.” Dinenympha, however, represents a totally 
different type, provided with four or eight peculiar flagellar cords and differing 
also in other important characters. 
