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Intestinal Protozoa in Termites 
(3) Family Holomastigotidae Grassi (emend.). 
Body not distinctly divided into different parts—as in Trichonympha. 
Flagella arranged in spiral rows from their commencement at anterior end; 
direction of spiral dexiotropic. A part of the hind end of the body—which 
may be large or small—free from flagella. A peculiar mass of dense protoplasm 
exists at the anterior portion of the body, and surrounds the nucleus, but in 
some genera this mass is less dense in the immediate vicinity of the nucleus. 
A tubular organ in connexion with this structure may or may not be present. 
Surface of the body ridged or apparently smooth. Nucleus single, vesicular. 
No visible mouth. Multiplication by simple longitudinal division, with mitosis 
of nucleus. No cysts or sexual stages known. 
Genus I. Holomastigotes Grassi, 1892. 
Small forms. Surface of the body with spiral ridges, few in number. Basal 
granules of flagella situated at the bottom of the groove behind each ridge, 
the basal parts of the flagella being fixed on the surface of the ridges. A dense 
mass of protoplasm is present at the anterior end, the nucleus being embedded 
in it. Nucleus anterior, spherical. Type species H. elongatum Grassi, 1892. 
(In Leucotermes ( Reticulitermes) lucifugus , Italy.) 
Genus II. Holomastigotoides Grassi, 1911. 
Large forms. Surface of the body with numerous spiral ridges, the flagella 
being attached as in the preceding genus. A mass of dense protoplasm is 
present at the anterior portion, and distinctly developed: it surrounds and 
sometimes extends behind the nucleus. Nucleus anterior, ovoid or compressed 
antero-posteriorly. Type species H. hertwigi (Hartmann) Grassi, 1911. (In 
Coptotermes hartmanni , Brazil.) 
Genus III. Spirotrichonympha Grassi, 1911. 
Medium-sized forms. The rows of basal granules of the flagella lie some¬ 
what more deeply in the endoplasm than in the preceding genera; being 
deepest at the anterior end and becoming more superficial towards the 
posterior. A fairly large part of the root of each flagellum is thus embedded 
in the protoplasm. The mass of dense protoplasm assumes the shape of a 
cone, its hinder border being indistinct. The spherical nucleus lies apparently 
free in the endoplasm, at some distance from the anterior extremity. Type 
species Sp. flagellata Grassi, 1892. (In Leucotermes ( Reticulitermes) lucifugus , 
Italy.) 
Genus IV. Microspironympha (gen. nov.). 
Small forms. Surface of the body not ridged: the rows of basal granules 
lie under the surface, but are somewhat more deeply placed at the anterior 
end. Nucleus spherical, anterior, but separated from the anterior extremity 
