HI tests of the 1968 plasmas incliided antigens of chikungunya, Sindbis, West 
Nile, Wesselsbron, yellow fever, Bunyamwera, Tahyna, Ingwavuma, Bahig, and 
Matruh, 
Neutralization tests were in baby mice i.c. using undiluted plasma or 
serum lln^ubated 1 hour at 37with decimal dilutions of virus and then inoc¬ 
ulated. Mice were observed usually for 2 weeks and end-points calculated by 
the Reed-Muench formula. 
For serological identification of isolates neutraliztion, HI, and 
complement-fixation (CF) tests were employed. 
Results 
Viruses isolated from birds . Viruses were isolated from migrating birds 
during both migration seasons of each year of this project. A total of 3,890 
pools or individual samples representing 6,152 bloods were processed from 
1966 through 1968. From these were isolated 54 mouse pathogenic agents. 
These are presumably viruses, although not all are completely characterized. 
Table 1 lists the viruses, their hosts, the dates of isolation, and results 
of re-isolation where attempted. Tables 2-8 list the bird species tested for 
virus isolation by season, year, and site of capture. 
In addition to the 3,890 samples above, 641 individual erythrocyte 
specimens collected during the fall of 1969 were inoculated into mice with 
all but 14 negative. Passages from these 14 bloods are frozen waiting de¬ 
finitive characterization. They include the following birds: Jynx torquilla 
(7985, 7990, 8011, 8292), Sylvia communis (8127, 8302), Sylvia curruca 
(8003, 8017), Caprimulgus europaeus (7986), Muscicapa striata (8029), 
Sylvia borin (80481), Phoenicurus phoenicurus (8056), Lanius minor (8293), 
and Oenanthe oenanthe (9516). 
68 
