the levels of 1:10 and 1:20. These viruses are all members of the Buny- 
amwera supergroup of arboviruses. Bahig virus did not react by HI test with 
64 other arboviruses. This is interpreted as evidence that Bahig virus per¬ 
tains to the Bunyamwera supergroup of arboviruses. 
Colonized Aedes aegypti were inoculated intra-thoracically with 10% 
mouse brain infected with Bahig virus. Virus was demonstrated in the mos¬ 
quito salivary glands 21 days later and 2 further serial passages in Aedes 
aegypti of infected salivary glands successful. Infected Aedes aegypti 
have been induced to take blood meals from baby mice, but these attempts 
have been unsuccessful in transmission of virus. 
Other isolates shown in Table 1 include E 890-3, closely related to the 
new Simbu group virus Thimiri, from a pool of four Lesser Whitethroat Warblers 
heading south on fall migration through Egypt; E 241 (probably not an arbo¬ 
virus) from a Red-backed Shrike, Lanius collurio , in Egypt in fall, C 40, 
E 3505, and E 3127, three related agents from a variety of spring and fall 
migrants from Cyprus and Egypt, which are not yet fully characterized; Ingwa- 
vuma, twice isolated from northward migrating Spotted Flycatchers, Musclcapa 
striata , in Cyprus in spring; 0 3255, related to Eg An 4996-63 isolated by 
Dr. Schmidt in Egypt during 1963, both viruses from viremic Turtle Doves, 
Streptopelia turtur, in Cyprus in spring; and West Nile virus from the Barred 
Warbler, Sylvia n isoria, also in Cyprus in spring. 
Serological results . HI tests with 16 arbovirus antigens were done on 868 
sera collected in Egypt during the fall of 1966. While most were migratory, a 
few were from indigenous water birds of the Nile delta. The antigens included 
6 group A viruses (Middelburg, Sindbis, Semliki, Y-62-33, chikungunya, Ndumu), 
8 group B viruses (Entebbe bat, yellow fever, Wesselsbron, Israel turkey Meningo¬ 
encephalitis, Zika, Ntaya, louping ill. West Nile), and Tahyna and Bunyamwera. 
70 
