^3574-5, This strain of West Nile virus was referred to Dr. James W. 
Ryan, Department of Epidemiology, School of Hygiene and Public Health, The 
Johns Hopkins University, for determination of geographic sub-type by the 
grid HI test. Dr. Ryan reported: "For testing West Nile strains, I used 
individual chick antisera, screened for specificity, in the grid HI test 
overnight at 4°C with a final pH of 7.0. In this test anti-Cyprus serum 
did not give a complete identity reaction with any other strain but was 
only two-fold different from strains in Africa south of the Sahara, slightly 
more different from those of the Near East and definitely different from those 
of Pakistan, France, India, and Nigeria. 
The most discriminatory southern African antiserum gave reactions of 
identity with the Cyprus isolate, as opposed to slight but definite differences with 
Near Eastern strains; and the.most discriminatory Near Eastern antiserum gave 
slight but definite differences with the Cyprus and southern African Isolates. 
From these results I would think that the Cyprus isolate was acquired from 
the region south of Egypt and probably south of the Sahara." 
Serological study of EgAnll69- 61 , Anl825-61 , Anl477-61 , An3782-62 , and 
Anl398-61 isolated by Dr. J. Schmidt of NAMRU-3 . 
EgAnll69-61 . This virus was isolated in 1961 from Phoenicurus phoenicurus . 
It has been identified as Kemerovo virus (recovered in 1962 from Ixodes persulcatus 
and from febrile humans in Westem-Siberia) on the basis of CF and neutralization 
tests (Table 19). 
EgAnl825-61 . This virus was isolated in 1961 from Phylloscopus trochilus . 
It has been identified as a new virus, related to Uukuniemi and Grand Arbaud. 
Uukuniemi was first recovered from Ixodes ricinus in 1959 in South western 
Finland and is believed to involve Ixodes and birds in its cycle. Ticks have 
also been implicated in the cycle of Grand Arbaud virus. Table 20 shows the 
relationships by CF of these viruses. 
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