194 
Journal of Agricultural Research 
Vol. I, No. 3 
OTHER PLANTS INOCULATED WITH THE ORGANISM FROM BOTH HOSTS 
That this bacterial spot is not confined to sugar-beet and nasturtium 
leaves has been shown by a number of inoculations performed upon 
other plants growing in the greenhouse. Both strains of the organism 
were used. Diseased spots were produced with the bacteria upon leaves 
of pepper, lettuce, eggplant, and upon the leaves and pods of the bean 
plant. Inoculatidn experiments were also tried on potato, clover, %nd 
daisy plants, but without any definite infection, although there was 
slight discoloration on potato leaves. 
The stems and leaves of the young pepper plants were readily in¬ 
fected through needle-prick inoculations. The spots were black, and the 
stems seemed more susceptible than the leaves. 
Lettuce leaves growing in the greenhouse blackened readily after in¬ 
oculation. One plant out of seven was entirely destroyed by the in¬ 
fection. One month later, when the temperature of the greenhouse was 
not so even throughout the day and night and the plants of the same 
lot had stopped growing rapidly and become toughened, the organism 
failed to produce infection. 
The leaves of eggplant were inoculated, and brown spotting resulted at 
the punctured places; later, these areas dropped out of the leaves. 
Of these various hosts the bean proved especially susceptible to the 
organism, inoculations taking effect almost as readily as upon the nas¬ 
turtium and sugar-beet leaves. Bean plants inoculated with a young 
agar culture of both strains of the organism showed the characteristic 
brown spots on the leaves within three to five days. Ten days after in¬ 
oculation some of the diseased leaves (PI. XIX, fig. i) were examined, and 
active bacteria were found in the cells. Three weeks after inoculation 
the bean leaves shriveled and died. Later, inoculations which were made 
upon the young pods of bean plants produced conspicuous, somewhat 
sunken, brownish spots in the tissue. (PI. XIX, fig. 3.) 
At the same time that the inoculation experiments were being carried 
on, cultural and morphological studies were made with both strains in the 
laboratory. From time to time notes and various tests were compared, 
and, as a result, the identity of the two strains was established. Such 
being the case, only one description will hereafter be given for the two 
strains, except where marked differences occur. 
DESCRIPTION OF THE ORGANISM 
MORPHOLOGICAL CHARACTERS 
Vegetative Cells. —The organism is a medium-sized schizomycete of 
varying length when grown in different media. It is a short rod with 
rounded ends, occurring singly or in pairs (fig. 1); occasionally it pccurs 
in long chains of two to many elements and again in long unsegmented 
