198 
Journal of Agricultural Research 
Vol. I, No. 3 
Bouillon Containing Sodium Chlorid. —Growth occurred in neutral 
bouillon containing 5 per cent of sodium chlorid when tests were made 
with the organism soon after first isolating. Three years later these 
tests were repeated. Growth then took place in bouillon containing 3 
and 3X per cent of sodium chlorid, but there was no growth in bouillon 
to which 4 per cent of sodium chlorid was added. 
Bouillon Over Chloroform.—T here is retardation of growth for two 
days; then the bouillon clouds and in nine days is colored a yellowish 
green tinge, as in the +15 bouillon without chloroform. 
Nitrate-Bouillon Cultures. —In nitrate bouillon a thin clouding is 
produced within 24 hours, and in four days the solution is distinctly 
clouded, especially in its upper portion, where pseudozooglceaelike masses 
are visible. In eight days the thin pellicle which forms on the surface 
is easily shaken into many small particles. At this time a slight greenish 
cast appears in the solution. The same ropelike sediment described in 
beef bouillon was observed in a 9-week’s-old culture of nitrate bouillon. 
Uschinsky’s Solution. —In plain Uschinsky’s solution and in the 
peptonized solution (1 per cent) strong clouding was produced in three 
to five days. In four days a thin pellicle composed of pseudozooglceae¬ 
like masses was observed. A greenish fluorescence became visible in 
five to eight days, and in three weeks the uniformly clouded solution 
had turned pale green (No. 328B, Code des Couleurs, Klincksieck et 
Valette). 
Fermi’s Solution. —There is a slight clouding in one day. In five 
days there is a thick tenacious pellicle, and the medium has changed to 
a decided pea-green color. A few fragments on the underside of the 
pellicle are suspended in the medium, and these occur in long gelatinous 
strings. On shaking the culture it is difficult to break up the pellicle 
and cause it to sink. In one month this pellicle is from 3 to 4 mm, 
thick. 
Cohn’s Solution.— The organism does not grow in Cohn’s solution. 
Sterile Milk. —The milk is cleared slightly in two to four days, 
showing a gradual separation of whey from curd. This separation 
begins on the surface as a watery band and gradually extends downward, 
becoming complete in 12 days when kept at room temperature from 18 
to 22 0 C. The medium is a yellowish cream color with a suggestion of 
green. There is a slight rim, but no pellicle. In one month the medium 
has become darker, and the green tinge has disappeared. It is trans¬ 
lucent throughout. Compared with Ridgway’s Color Chart, it is a clay 
color. After two months at room temperature the cultures are dried 
down 5 c. c., and are of a thick, creamy consistency. Transfers from 
these cultures showed that the organism was still alive. 
Litmus Milk. —In two days a blue ring appears at the surface of the 
liquid, extending down about 1 cm. In four days there are three rings 
