Dec, io, 1913 
Disease of Sugar-Beet and Nasturtium Leaves 
199 
of graded shades of blue, while the lowest third of the liquid remains the 
color of the check tubes. Six to eight days later none of the original color 
of the liquid remains. Some tubes have four or five rings of color, the 
upper ring being the darkest blue. From 12 to 15 days after inoculating 
a brownish color appears at the bottom of the tube and extends up¬ 
ward, changing the entire liquid to a muddy blue in from three to six 
days. About four days later the medium begins to change to blue again 
and in seven days is entirely blue, approaching Ridgway’s plum purple. 
Four different tests were made in which the color changes followed in 
this same manner. Room temperature, 18 0 to 22 0 C. 
Gelatin Plates. —Colonies of the bacterium which appear on gelatin 
( + 10) plates within 48 hours are whitish, round, and glistening, with a 
smooth, flat surface having fishscalelike markings. Slight liquefaction 
began in two days at a temperature of 20° to 22 0 C., causing small clear 
areas around the colonies. In thickly sown plates liquefaction pro¬ 
ceeded rapidly, becoming complete in three to five days. In plates 
thinly sown the liquefaction is only in cuplike areas about the colonies. 
When liquefied, the gelatin becomes a turbid, slightly greenish fluid. 
Gelatin Stab Cultures. —In gelatin ( + 10) stabs, growth was visible 
in two days on the surface about the stab, extending downward about 
1 cm. (temperature 20° to 22 0 C.). Craterlike depressions with fluid 
contents were observed on the third day, increasing in size until a layer 
of fluid was formed. In 10 days this layer had become 1 cm. in depth. 
Liquefaction of the gelatin stab culture was complete in 30 days. 
Steamed Potato Cylinders, —In three days growth on this medium 
is abundant, flat, smooth, cream white, and glistening. The potato 
changes to a gray-brown color in 3 days, and in 15 days is from two 
to four shades darker. The bacterial slime approaches Ridgway’s wood 
brown. There is no diastasic action of the starch. 
Starch Jelly. —Growth is scant on starch jelly. In seven days the 
medium at the surface and about 3 mm. below the streak along which 
the growth of the organism has taken place has changed to a delicate 
green. The test for sugar with Fettling’s solution was negative. 
LoEFRlER's Blood Serum. —The growth is moderate and slow, scarcely 
a trace occurring in three days. The medium becomes gray and at the 
end of 32 days has liquefied a little. The stroke is filiform, flat, glisten¬ 
ing, and smooth. The heaviest growth occurs in the condensation 
water. 
Litmus-Lactose Agar. —Copious growth developed within two 
weeks in litmus-lactose agar cultures. The condensation water first 
clouded, after which growth began to show at the base of the stroke. In 
eight days there was growth along the entire stroke, with a spreading 
at the base and a pellicle formation in the condensation water. The 
medium was blued. At the end of nine weeks the growth was azure 
blue in color (No. 401, Code des Couleurs, Klincksieck et Valette). 
