202 
Journal of Agricultural Research 
Vol. I, No. 3 
Gas Formation. —The organism is aerobic and does not form gas. 
Tests were made in fermentation tubes with water containing 2 per 
cent of Witte’s peptone to which was added 1 per cent of each of the 
following carbon compounds: Glycerin, saccharose, mannite, maltose, 
dextrose, and lactose. (Levulose and galactose were used in addition 
with the strain of the organisms isolated from nasturtium.) No gas 
formed in any of the tubes. Because of differences between the two 
strains in regard to the clouding of solutions in the closed end of some 
of the fermentation tubes, the results of the tests are given separately. 
With the organism isolated from sugar beet there was a heavy growth 
in the open arm of the tubes, but none in the closed ends. Dextrose 
and saccharose gave an acid test with litmus after the organism had 
been growing in the tubes 16 days. Glycerin, mannite, maltose, and 
lactose gave an alkaline test. 
From inoculations with the organism isolated from nasturtium the 
following readings were made after 5, 10, and 28 days: 
Table I .—Readings from fermentation tubes inoculated with the nasturtium strain of the 
bacterium. 
1 
Peptonized 
water with 
per cent solu¬ 
tion of— 
After 5 days. 
After 10 days. 
After 28 days. 
Lactose.. 
Levulose 
Maltose.. 
Solution clouded 
in open end. 
— do... 
_do. 
Mannite 
do 
Glycerin 
do 
Dextrose 
do 
Clouded in open end and outer 
two-thirds of U tube; sharp line 
of demarcation; perfectly clear 
in closed end; no pellicle; litmus 
test, alkaline. 
Clouded in open end and outer 
two-thirds of U tube; clear in 
closed end; no pellicle and no 
flocculence; litmus test, alkaline. 
Uniformly clouded in open end 
and outer two-thirds of U tube; 
sharp line of demarcation; no 
pellicle; clear in closed end; lit¬ 
mus test, alkaline. 
Clouded in open end and in U 
tube; no sharp line of demarca¬ 
tion; no pellicle; perfectly clear 
in closed end; litmus test, alka¬ 
line. 
Uniformly clouded in open end 
and outer two-thirds of U tube; 
sharp line of demarcation; no 
pellicle; clear in closed end; lit¬ 
mus test, alkaline. 
Uniform clouding in open end and 
whole of U tube; no pellicle; 
clear in closed end; litmus test, 
acid. 
Galactose. 
do 
Clouded in open end and in U 
tube; no distinct line of demar¬ 
cation; faint clouding in closed 
end; no pellicle; litmus test, dis¬ 
tinctly acid. 
Saccharose. 
do 
Uniformly clouded in open end 
and in U tube; no sharp line of 
demarcation; no pellicle; clear in 
closed end; litmus test, feebly 
acid. 
Clouded in open end and outer 
part of U tube; whitish pre¬ 
cipitate; no growth in closed 
end; litmus test, alkaline. 
Clouded in open end and outer 
U tube; clear in closed end; 
litmus test, alkaline. 
Clouded in open end, with 
whitish precipitate; no 
growth in closed end; litmus 
test, alkaline. 
Clouded in open end and U 
tube; clear in closed end; no 
pellicle; whitish precipitate; 
litmus test, alkaline. 
Clouded in open end, with 
whitish precipitate; clear in 
closed end; litmus test, alka¬ 
line. 
Well clouded in open end, with 
numerous small particles in 
suspension; closed end clear, 
except a slight clouding in 
lower end; no pellicle; litmus 
test, distinctly acid. 
Well clouded in open end, with 
many small particles in sus¬ 
pension; clouded in two- 
thirds of closed end; no pel¬ 
licle; considerable precipi¬ 
tate; litmus test, distinctly 
acid. 
Thinly and uniformly clouded 
in open end and outer two- 
thirds of U tube; sharp line of 
demarcation; clear in closed 
end; no pellicle; whitish pre¬ 
cipitate; litmus test, dis¬ 
tinctly add. 
