2 l6 
Journal of Agricultural Research 
Vol. I, No. 3 
When not under observation, each slide was placed in a folder of dark 
paper which left only one edge exposed, and was filed with others of the 
same experiment in a shallow box constructed for the purpose. 
Observations were as a rule made twice daily, in the early morning 
and in the late afternoon, the intervening time being considered, for the 
purposes of the notes made, as half a day. 
It was found that a living worm within its cocoon would respond 
immediately to the stimulus if a needle was thrust through the bottom 
of the cocoon. This aided materially in the determination of the time 
at which oviposition of the parasite took place, since, with but one excep¬ 
tion, the parasite was never known to deposit an egg without first killing 
the host larva. 
The food supplied the parasites consisted of sweet liquids, such as 
sugar solution, dilute molasses, and strained honey. All of these sub¬ 
stances were lapped up greedily by the parasites of both sexes. 
REPRODUCTION 
THE EXTERN AT SEXUAE APPARATUS 
Ovipositor. —The ovipositor (figs, i, 2, and 3) is composed of five 
long slender pieces. The two outer ones are black and hairy, grooved 
longitudinally within, and form a tube or 
sheath surrounding the ovipositor proper. 
Next inside of this is a smooth chitinized 
piece, deeply grooved on the ventral side and 
terminating in a prowlike point. At its base 
it is forked, indicating that it is formed of 
two opposed pieces fused along their dorsal 
edges. Within this is a pair of very slender 
flattened pieces barbed at their tips. 
The outside pair together form the sheath. 
This has no part in the act of oviposition, 
but is merely a protection for the ovipositor 
proper, which is composed of the three other 
pieces. The single piece may be called the 
“lance,” since it is with this that the host 
larva is pierced. The inner pair have been 
variously termed “lancets,” “stylets,” etc. 
In oviposition the egg passes down the channel fig. 2.—Caihephiaiies <s P . : lateral 
formed by the three parts of the ovipositor view of terminal abdominal seg¬ 
ments, showing relative position 
proper. of elements of ovipositor, a, Valves 
On each side and slightly above the base of sheath: b > lance = lancets; d, 
cerci. 
of the sheath is a small tuberclelike appendage 
bearing a number of long, stiff hairs. These are the cerci. 
GeniTaeia of MaeE. —The male external sexual organs (figs. 4 and 5) 
consist of two sets of paired pieces and the penis. The outer pair are 
c 
Fig. 1—Colliephialtes sp.: Ventral 
view of terminal'abdominal seg¬ 
ments, showing relative position of 
elements of ovipositor, a, Valves 
of sheath; b , lance; c, lancets; d, 
cerci. 
