270 
Journal of Agricultural Research 
Vol. I, No. 3 
It is seen from Table III and also from figure 2 that the temperatures 
of thermostats I, II, III, and V (1.09 0 to 9.0 0 C.) are prohibitive of growth. 
A sparse growth took place in thermostat VI (11.2 0 to 14,0° C.) and 
reached its maximum growth in the laboratory room (17.2 0 to 24. 5 0 C.). 
The best growth was obtained at an average temperature of 21.9 0 C. and 
the next best in thermostat XI (29.4 0 to 30.8° C.). The growth of mycelia 
in thermostat XI (29.4 0 to 30.8° C.) was better at the outset than at any 
other temperature, although the production of pycnidia and spores was 
not as good at the end of the experiment as in cultures growing in the 
laboratory room. The medium in thermostat XI was decidedly dis¬ 
colored, a change which did not occur at any other temperature. In 
AOS 4.S 7i€ 3.0 /as /5<2 SG4 /7S 2/3 302 373 
thermostat XII (36.4° to 38.0° C.) a very slight growth of hyphae took 
place during the first three or four days. No further development took 
place thereafter. While these results do not definitely fix the limiting 
temperature for growth, they show that the optimum probably lies some¬ 
where between 21.9 0 and 30.2 0 C., the minimum close to 12.6° C., and the 
maximum at about 37.3 0 C. 
At the conclusion of the incubation period all the cultures were taken 
from the thermostats and placed on a table in the laboratory room. At 
the end of 10 days there was a good growth in all the tubes except in 
those that were in thermostat XII (36.4° to 38.0° C.), cultures of which 
had been killed in 21 days. 
